Negative regulation of T-cell adhesion and activation by CD43

CD43 is a cell-surface sialoglycoprotein expressed by a variety of haematopoietically derived cells, including T lymphocytes 1á¤-9 . Earlier observations of defective CD43 expression by ? lymphocytes from boys with the X-chromosome-linked Wiskottá¤-Aldrich syndrome suggested the importance of CD43 i...

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Veröffentlicht in:Nature (London) 1995-10, Vol.377 (6549), p.535-539
Hauptverfasser: Manjunath, N, Correa, Mariangela, Ardman, Margarett, Ardman, Blair
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Sprache:eng
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Zusammenfassung:CD43 is a cell-surface sialoglycoprotein expressed by a variety of haematopoietically derived cells, including T lymphocytes 1á¤-9 . Earlier observations of defective CD43 expression by ? lymphocytes from boys with the X-chromosome-linked Wiskottá¤-Aldrich syndrome suggested the importance of CD43 in lymphocyte function 10,11 . Subsequent studies have suggested that CD43 facilitates leukocyte adhesion 12á¤-14 and has a co-stimulatory role during T-cell activation 15 . To define the physiologically relevant function(s) of CD43, we have generated CD43-knockout mice. We report here that CD43-deficient T cells from such mice show a marked increase in their in vitro proliferative response to concana-valin A, anti-CD3, the superantigen SEB and allostimulation. Additionally, CD43-deficient ? cells show a substantial enhancement in homotypic adhesion and in their ability to bind different ligands, including fibronectin and the intercellular adhesion molecule ICAM-1. Vaccinia-virus-infected CD43-knockout mice mounted an augmented anti-vaccinia cytotoxic T-cell response compared with their wild-type littermates, yet developed an increased virus load. We conclude that CD43 negatively regulates T-cell activation and adhesion and is important for viral clearance.
ISSN:0028-0836
1476-4687
DOI:10.1038/377535a0