An Improved Extraction Procedure for an HPLC Method for Cyclosporin a in Whole Blood and Plasma

An Improved Extraction Procedure for an HPLC Method for Cyclosporin a in Whole Blood and Plasma

Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on th...

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Veröffentlicht in:Annals of Clinical Biochemistry 1987-03, Vol.24 (2), p.161-166
Hauptverfasser: Maguire, S, Kyne, F, Uaconaill, D
Format: Artikel
Sprache:eng
Schlagworte:
Acetates
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cyclosporins - blood
Ether
Hexanes
Humans
Immunomodulators
Medical sciences
Pharmacology. Drug treatments
Solvents
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Zusammenfassung:Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.
ISSN:0004-5632
1758-1001
DOI:10.1177/000456328702400206