Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis
We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and pa...
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| Veröffentlicht in: | Plant molecular biology 1995-09, Vol.28 (6), p.1133-1141 |
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| container_title | Plant molecular biology |
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| creator | Osakabe, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry) Osakabe, K Kawai, S Katayama, Y Morohoshi, N |
| description | We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes. |
| doi_str_mv | 10.1007/bf00032674 |
| format | Article |
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(Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry) ; Osakabe, K ; Kawai, S ; Katayama, Y ; Morohoshi, N</creator><creatorcontrib>Osakabe, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry) ; Osakabe, K ; Kawai, S ; Katayama, Y ; Morohoshi, N</creatorcontrib><description>We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. 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(Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry)</creatorcontrib><creatorcontrib>Osakabe, K</creatorcontrib><creatorcontrib>Kawai, S</creatorcontrib><creatorcontrib>Katayama, Y</creatorcontrib><creatorcontrib>Morohoshi, N</creatorcontrib><title>Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.</description><subject>Amino Acid Sequence</subject><subject>AMONIO FENILALANINA LIASA</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA Primers - chemistry</subject><subject>GENE</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>GENES</subject><subject>Genes, Plant</subject><subject>Introns</subject><subject>MESSENGER RNA</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>PHENYLALANINE AMMONIA LYASE</subject><subject>Phenylalanine Ammonia-Lyase - genetics</subject><subject>Plant Proteins - genetics</subject><subject>POPULUS</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - genetics</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Trees - enzymology</subject><subject>Trees - genetics</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP3DAQha0KBMu2lx6RKvnEASlgr-M4OcKqtEgrqKr2HE2cMetix4udVFp-Q380rnbhymmkN997Gs0j5DNnF5wxddkZxphYVKr8QGZcKlFItqgPyIzxShVlyRfH5CSlP4xlXFRH5EjJsq4Fn5F_yzVE0CNG-wyjDQMNho5rpGmMkx6niBSGnvaYCW-HN8T_vLuiDv-iS6-OzRqHrQMHgx2yy_swWCjcFhLSB8ySAW_dlpoYPP0RNpObEn20IzxmHYdk00dyaMAl_LSfc_L75uuv5fdidf_tdnm1KrSQaiy6UteNBC5AIK_AaFP2EpoGmt4wIwVWtRCKa92VdVawYQp53XXYmH5RgRBzcrbL3cTwNGEaW2-TRpdPxzClVikpaiWqd0Gu8kulUBk834E6hpQimnYTrYe4bTlr_3fUXt-8dpThL_vUqfPYv6H7UvL-dLc3EFp4iDa1d6tGMtnkal8ARxWXkA</recordid><startdate>199509</startdate><enddate>199509</enddate><creator>Osakabe, Y. 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Faculty of Agriculture, Lab. of Wood Chemistry) ; Osakabe, K ; Kawai, S ; Katayama, Y ; Morohoshi, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-b4c895a13a3e16afcf4d5a99a9df0f53e683371ccb48df0e907e18bbe9fd26a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>AMONIO FENILALANINA LIASA</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA Primers - chemistry</topic><topic>GENE</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>GENES</topic><topic>Genes, Plant</topic><topic>Introns</topic><topic>MESSENGER RNA</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>PHENYLALANINE AMMONIA LYASE</topic><topic>Phenylalanine Ammonia-Lyase - genetics</topic><topic>Plant Proteins - genetics</topic><topic>POPULUS</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - genetics</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Trees - enzymology</topic><topic>Trees - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Osakabe, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry)</creatorcontrib><creatorcontrib>Osakabe, K</creatorcontrib><creatorcontrib>Kawai, S</creatorcontrib><creatorcontrib>Katayama, Y</creatorcontrib><creatorcontrib>Morohoshi, N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Osakabe, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry)</au><au>Osakabe, K</au><au>Kawai, S</au><au>Katayama, Y</au><au>Morohoshi, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1995-09</date><risdate>1995</risdate><volume>28</volume><issue>6</issue><spage>1133</spage><epage>1141</epage><pages>1133-1141</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.</abstract><cop>Netherlands</cop><pmid>7548831</pmid><doi>10.1007/bf00032674</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1995-09, Vol.28 (6), p.1133-1141 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence AMONIO FENILALANINA LIASA ARN MENSAJERO ARN MESSAGER Base Sequence Cloning, Molecular DNA Primers - chemistry GENE Gene Expression Gene Expression Regulation, Plant GENES Genes, Plant Introns MESSENGER RNA Molecular Sequence Data NUCLEOTIDE SEQUENCE PHENYLALANINE AMMONIA LYASE Phenylalanine Ammonia-Lyase - genetics Plant Proteins - genetics POPULUS Promoter Regions, Genetic RNA, Messenger - genetics SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Trees - enzymology Trees - genetics |
| title | Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis |
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