Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis

Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis

We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and pa...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant molecular biology 1995-09, Vol.28 (6), p.1133-1141
Hauptverfasser: Osakabe, Y. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry), Osakabe, K, Kawai, S, Katayama, Y, Morohoshi, N
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
AMONIO FENILALANINA LIASA
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Cloning, Molecular
DNA Primers - chemistry
GENE
Gene Expression
Gene Expression Regulation, Plant
GENES
Genes, Plant
Introns
MESSENGER RNA
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
PHENYLALANINE AMMONIA LYASE
Phenylalanine Ammonia-Lyase - genetics
Plant Proteins - genetics
POPULUS
Promoter Regions, Genetic
RNA, Messenger - genetics
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Trees - enzymology
Trees - genetics
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We isolated two new PAL genes, palg2b and palg4, from Populus kitakamiensis, palg2a and palg2b are clustered and palg2b encodes a polypeptide of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many phenylpropanoid biosynthetic genes. We measured the mRNA levels of the four PAL genes by S1 mapping using total RNA from stem tissues developing secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00032674