Rapid diagnosis of herpes simplex virus infections in conventional and shell vial cell cultures using monoclonal antibodies

One hundred specimens for herpes simplex virus (HSV) isolation were tested in parallel by conventional and by centrifugation-enhanced cell culture, followed by identification using monoclonal antibodies to HSV-1 and HSV-2. Sensitivity was comparable by the two methods; conventional culture was only...

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Veröffentlicht in:	Journal of virological methods 1987-03, Vol.15 (4), p.329-330
Hauptverfasser:	Winter, G.F., Inglis, J.M., Cubie, Heather A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Biological and medical sciences Cells, Cultured Centrifugation Centrifugation enhancement Culture Techniques - instrumentation Fundamental and applied biological sciences. Psychology Herpes Simplex - diagnosis Herpes Simplex - immunology Herpes simplex virus herpes simplex virus 1 herpes simplex virus 2 Humans Microbiology Monoclonal antibody Simplexvirus - immunology Techniques used in virology Time Factors Virology
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Beschreibung
Zusammenfassung:	One hundred specimens for herpes simplex virus (HSV) isolation were tested in parallel by conventional and by centrifugation-enhanced cell culture, followed by identification using monoclonal antibodies to HSV-1 and HSV-2. Sensitivity was comparable by the two methods; conventional culture was only marginally slower and was easier to fit into the routine of a busy laboratory. It is. therefore, advocated for HSV detection in clinical specimens.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(87)90156-X