Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography
A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide var...
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| Veröffentlicht in: | Analytical biochemistry 1987-02, Vol.160 (2), p.388-391 |
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| container_end_page | 391 |
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| container_title | Analytical biochemistry |
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| creator | Pohl, Thomas Kamp, Roza Maria |
| description | A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection. |
| doi_str_mv | 10.1016/0003-2697(87)90065-0 |
| format | Article |
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The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(87)90065-0</identifier><identifier>PMID: 3034092</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cattle ; Chromatography, High Pressure Liquid - methods ; Cytochrome c Group - isolation & purification ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; high-performance liquid chromatography ; microsequencing ; Muramidase - isolation & purification ; protein concentration ; protein desalting ; Proteins ; Proteins - isolation & purification ; reversed-phase HPLC ; ribosomal proteins ; Salts - isolation & purification ; Serum Albumin, Bovine ; Solutions</subject><ispartof>Analytical biochemistry, 1987-02, Vol.160 (2), p.388-391</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-750527f149697c49d83cb1bc1e7e39394a58aeeaae36c878fbf45dc494c27e243</citedby><cites>FETCH-LOGICAL-c417t-750527f149697c49d83cb1bc1e7e39394a58aeeaae36c878fbf45dc494c27e243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(87)90065-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7491266$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3034092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pohl, Thomas</creatorcontrib><creatorcontrib>Kamp, Roza Maria</creatorcontrib><title>Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cytochrome c Group - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>high-performance liquid chromatography</subject><subject>microsequencing</subject><subject>Muramidase - isolation & purification</subject><subject>protein concentration</subject><subject>protein desalting</subject><subject>Proteins</subject><subject>Proteins - isolation & purification</subject><subject>reversed-phase HPLC</subject><subject>ribosomal proteins</subject><subject>Salts - isolation & purification</subject><subject>Serum Albumin, Bovine</subject><subject>Solutions</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhC0EWoaFNwDJB4TgEGjHjh1fkNDyK63EBc6Wx-lMjJI4azsj7dvj7IzmCKc-1Fel7mpCXjJ4z4DJDwDAq1pq9bZV7zSAbCp4RHYMtKyAg35MdhfkKXmW0h8AxkQjr8gVBy5A1zty_IzJjtnPB2rnjrowO5xztNmHmYaeLjFk9HOifqadH9eMNIUyNnlNmy3iEWPCrloGm5AO_jBUC8Y-xMmWMDr6u9WX5CGGyeZwiHYZ7p-TJ70dE744z2vy--uXXzffq9uf337cfLqtnGAqV6qBplY9E7rc4ITuWu72bO8YKuSaa2Gb1iJai1y6VrX9vhdNV0DhaoW14NfkzSm33HG3Yspm8snhONoZw5qMUk3dSvF_8GEFKaGA4gS6GFKK2Jsl-snGe8PAbH8xW-lmK920yjz8xWy2V-f8dT9hdzGdH1H012fdJmfHPpbufLpgSmhWS1mwjycMS2lHj9Ek57HU3PmILpsu-H_v8ReRAquc</recordid><startdate>19870201</startdate><enddate>19870201</enddate><creator>Pohl, Thomas</creator><creator>Kamp, Roza Maria</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19870201</creationdate><title>Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography</title><author>Pohl, Thomas ; Kamp, Roza Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-750527f149697c49d83cb1bc1e7e39394a58aeeaae36c878fbf45dc494c27e243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cytochrome c Group - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>high-performance liquid chromatography</topic><topic>microsequencing</topic><topic>Muramidase - isolation & purification</topic><topic>protein concentration</topic><topic>protein desalting</topic><topic>Proteins</topic><topic>Proteins - isolation & purification</topic><topic>reversed-phase HPLC</topic><topic>ribosomal proteins</topic><topic>Salts - isolation & purification</topic><topic>Serum Albumin, Bovine</topic><topic>Solutions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pohl, Thomas</creatorcontrib><creatorcontrib>Kamp, Roza Maria</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pohl, Thomas</au><au>Kamp, Roza Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1987-02-01</date><risdate>1987</risdate><volume>160</volume><issue>2</issue><spage>388</spage><epage>391</epage><pages>388-391</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3034092</pmid><doi>10.1016/0003-2697(87)90065-0</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1987-02, Vol.160 (2), p.388-391 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77528644 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Chromatography, High Pressure Liquid - methods Cytochrome c Group - isolation & purification Fundamental and applied biological sciences. Psychology General aspects, investigation methods high-performance liquid chromatography microsequencing Muramidase - isolation & purification protein concentration protein desalting Proteins Proteins - isolation & purification reversed-phase HPLC ribosomal proteins Salts - isolation & purification Serum Albumin, Bovine Solutions |
| title | Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography |
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