Desalting and concentration of proteins in dilute solution using reversed-phase high-performance liquid chromatography

A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide var...

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Veröffentlicht in:	Analytical biochemistry 1987-02, Vol.160 (2), p.388-391
Hauptverfasser:	Pohl, Thomas, Kamp, Roza Maria
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Chromatography, High Pressure Liquid - methods Cytochrome c Group - isolation & purification Fundamental and applied biological sciences. Psychology General aspects, investigation methods high-performance liquid chromatography microsequencing Muramidase - isolation & purification protein concentration protein desalting Proteins Proteins - isolation & purification reversed-phase HPLC ribosomal proteins Salts - isolation & purification Serum Albumin, Bovine Solutions
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Beschreibung
Zusammenfassung:	A rapid method for desalting and concentrating dilute protein solutions using short reversed-phase columns (3–4 cm) has been described. The recovery of proteins is usually 90–100%. The method is simple and rapid and allows the desalting and concentration of protein samples simultaneously. A wide variety of proteins in the range up to 80 kDa can be desalted in microgram to milligram amounts, and volumes up to 1 liter can be concentrated to a few milliliters by a single injection.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(87)90065-0