Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions

Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O 2 −. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigator...

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Veröffentlicht in:	Analytical biochemistry 1987-03, Vol.161 (2), p.559-566
Hauptverfasser:	Beyer, Wayne F., Fridovich, Irwin
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle Cytochrome c Group Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Nitroblue Tetrazolium Oxidoreductases Superoxide Dismutase - analysis Xanthine Oxidase
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Beschreibung
Zusammenfassung:	Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O 2 −. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(87)90489-1