Lactobacillus protoplast transformation

Lactobacillus protoplast transformation

A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of prot...

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Veröffentlicht in:Plasmid 1987, Vol.17 (1), p.73-75
Hauptverfasser: Morelli, L., Cocconcelli, P.S., Bottazzi, V., Damiani, G., Ferretti, L., Sgaramella, V.
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
DNA
DNA, Bacterial - genetics
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Genetic technics
Lactobacillus
Lactobacillus - genetics
Methods. Procedures. Technologies
Mutant screening
Plasmids
Polyethylene Glycols
Prokaryotes
protoplasts
transformation
Transformation, Genetic
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Zusammenfassung:A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
ISSN:0147-619X
1095-9890
DOI:10.1016/0147-619X(87)90013-8