Molecular Cloning of ID4, a Novel Dominant Negative Helix-Loop-Helix Human Gene on Chromosome 6p21.3-p22

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate the expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent upon formation of homo- and/or heterodimers. Dominant negative HLH proteins (Id-...

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Veröffentlicht in:Genomics 1995-05, Vol.27 (1), p.200-203
Hauptverfasser: Pagliuca, Alfredo, Bartoli, Paola Cannada, Saccone, Salvatore, Della Valle, Giuliano, Lania, Luigi
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Sprache:eng
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Zusammenfassung:Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate the expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent upon formation of homo- and/or heterodimers. Dominant negative HLH proteins (Id-related genes) also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins. We report here the cDNA sequence of a novel human HLH gene (HGMW-approved symbol ID4) that lacks the basic domain. ID4 is differentially expressed in adult organs in four mRNA molecules, which are presumably a result of differential splicing and/or alternative usage of the polyadenylation sites. Transfection experiments indicated that enforced expression of Id-4H protein inhibits the trans -activation of the muscle creatine kinase E-box enhancer by MyoD. Finally, we localized the ID4 gene to the chromosome 6p21-p22 region.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1995.1026