Decrease of NCAM expression and astrocyte-neurone interaction in long-term cultured astrocytes

In order to assess the characteristics of the older astrocyte, we obtained long-term cultured rat astrocytes (20 months) and examined the features of protein expression in relation to neuronal interaction. In short-term cultured astrocytes, NCAM expressed strongly in contrast to weak expression of l...

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Veröffentlicht in:Neuroreport 1995-05, Vol.6 (8), p.1085-1088
Hauptverfasser: YAZAKI, T, ASOU, H, ARIMOTO, K, TOYA, S, UYEMURA, K
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Sprache:eng
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Zusammenfassung:In order to assess the characteristics of the older astrocyte, we obtained long-term cultured rat astrocytes (20 months) and examined the features of protein expression in relation to neuronal interaction. In short-term cultured astrocytes, NCAM expressed strongly in contrast to weak expression of laminin by both immunocytochemical and ELISA assay. On the contrary, in long-term cultured astrocytes, a marked decrease of NCAM expression was observed along with increased laminin expression compared with short-term cultured astrocytes. The long-term cultured astrocytes remained positive to anti-GFAP antibody and showed a much lower ability to interact with neurones than the short-term cultured astrocytes. NCAM may be one of the responsible molecules related to the astrocyte-neurone interaction in the developing and ageing nervous system.
ISSN:0959-4965
1473-558X
DOI:10.1097/00001756-199505000-00003