Serodiagnosis of new world Leishmaniasis by using a genus-specific antigen in enzyme linked immunosorbent assays

A genus-specific monoclonal antibody (83L-5G9), generated against promastigotes of Leishmania braziliensis panamensis (WRAIR-470), has been used as a ligand in immunochromatography columns for the recovery and purification of the genus-specific antigen. When the purified polypeptide, actually a doub...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Diagnostic microbiology and infectious disease 1987-03, Vol.6 (3), p.229-238
Hauptverfasser:	Sacci, John B., Christensen, Howard A., Vasquez, A., Anthony, Ronald L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - analysis Antibodies, Monoclonal - immunology Antigens, Protozoan - immunology Antigens, Protozoan - isolation & purification Antigens, Surface - immunology Antigens, Surface - isolation & purification Biological and medical sciences Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Human protozoal diseases Humans Immunologic Techniques Infectious diseases Leishmania - immunology Leishmania braziliensis - immunology Leishmaniasis - diagnosis Leshmaniasis Medical sciences Parasitic diseases Protozoal diseases Species Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A genus-specific monoclonal antibody (83L-5G9), generated against promastigotes of Leishmania braziliensis panamensis (WRAIR-470), has been used as a ligand in immunochromatography columns for the recovery and purification of the genus-specific antigen. When the purified polypeptide, actually a doublet comprised of a 58 kd and a 31 kd moiety, was used as the antigen in enzyme linked immunosorbent assays (ELISA), it was reactive with 36 of 85 sera from Leishmania patients and with 1 of 49 sera from confirmed cases of Chagas' disease. An additional 25 sera from an unexposed population and five specimens from individuals seropositive for Toxoplasma gondii were nonreactive.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/0732-8893(87)90017-4