Possible role of glutathione in cofactor regeneration of dopamine β-hydroxylase

The activity of the enzyme dopamine β-hydroxylase was determined in rat brain stem by a sensitive coupled radiometric assay. The appropriate copper and dilution parameters have been determined for this tissue. Reduced glutathione has been shown to activate the enzyme homogenate at concentrations of...

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Veröffentlicht in:	Biochemical medicine and metabolic biology 1987-02, Vol.37 (1), p.1-4
1. Verfasser:	OGUS, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Brain Stem - enzymology Copper - pharmacology Dopamine beta-Hydroxylase - antagonists & inhibitors Dopamine beta-Hydroxylase - metabolism Enzyme Activation - drug effects Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Glutathione - pharmacology Male Oxidoreductases Rats Rats, Inbred Strains
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Zusammenfassung:	The activity of the enzyme dopamine β-hydroxylase was determined in rat brain stem by a sensitive coupled radiometric assay. The appropriate copper and dilution parameters have been determined for this tissue. Reduced glutathione has been shown to activate the enzyme homogenate at concentrations of 24–240 μ m. This paper shows that glutathione cannot contribute to the inhibitory activity coming from the brain stem. A mechanism is proposed for the role of glutathione in cofactor regeneration of dopamine β-hydroxylase.
ISSN:	0885-4505 1557-7651
DOI:	10.1016/0885-4505(87)90002-8