Identification of the 190 KD Microtubule-Associated Protein in Cultured Fibroblasts and Its Association with Interphase and Mitotic Microtubules

We previously investigated the biochemical characteristics of microtubule-associated proteins (MAPs) of the adrenal medulla and adrenal cortex and found that they contain a new kind of MAP with a molecular weight of 190, 000 (190 kD MAP) as a major species (Kotani, S., H. Murofushi, S. Maekawa, C. S...

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Veröffentlicht in:Cell Structure and Function 1987, Vol.12(1), pp.1-9
Hauptverfasser: Kotani, Susumu, Murofushi, Hiromu, Maekawa, Shohei, Aizawa, Hiroyuki, Kaji, Kazuhiko, Sakai, Hikoichi
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Sprache:eng
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Zusammenfassung:We previously investigated the biochemical characteristics of microtubule-associated proteins (MAPs) of the adrenal medulla and adrenal cortex and found that they contain a new kind of MAP with a molecular weight of 190, 000 (190 kD MAP) as a major species (Kotani, S., H. Murofushi, S. Maekawa, C. Sato, and H. Sakai. Eur. J. Biochem. 156, 23-29, 1986). We now have used an affinity purified anti-(190 kD MAP) antibody and show by indirect immunofluorescent microscopy the association of this MAP with microtubules in situ in TIG-3 cells (human embryonic lung fibroblasts). The 190 kD MAP was present along the interphase and mitotic microtubules, and there was no marked difference between the staining pattern with anti-tubulin and that with anti-(190 kD MAP) antibodies, evidence that the localization of 190 kD MAP is not restricted to the subset of microtubules. We also isolated MAPs from TIG-3 cells and identified their 190 kD MAP as a major heat-stable component. Several other unidentified polypeptides were recovered in the MAP fraction specifically.
ISSN:0386-7196
1347-3700
DOI:10.1247/csf.12.1