Uptake and Release of Ca2+ in Chemically Skinned Aortic Strips from Spontaneously Hypertensive (SHR) and Normotensive (WKY) Rats
The uptake and release of Ca2+ were studied in EGTA-skinned aortic strips from spontaneously hypertensive rats (SHR strain: SAP=191±5mmHg, n=27) and normotensive control rats (WKY strain: SAP=131±2mmHg, n=25). 45Ca uptake was measured as a function of time (0.5 to 30min.), at pCa 6.6, in the presenc...
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| Veröffentlicht in: | Japanese Heart Journal 1995, Vol.36(3), pp.377-388 |
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| creator | AIELLO, Ernesto A. GRASSI, Angela O. |
| description | The uptake and release of Ca2+ were studied in EGTA-skinned aortic strips from spontaneously hypertensive rats (SHR strain: SAP=191±5mmHg, n=27) and normotensive control rats (WKY strain: SAP=131±2mmHg, n=25). 45Ca uptake was measured as a function of time (0.5 to 30min.), at pCa 6.6, in the presence of 10mM of K oxalate. Skinned aortic strips of SHRs accumulated more Ca2+ after 30min of uptake than those of WKY rats (0.66±0.05 vs 0.52±0.03nmole.mg-1 wet tissue; p |
| doi_str_mv | 10.1536/ihj.36.377 |
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Skinned aortic strips of SHRs accumulated more Ca2+ after 30min of uptake than those of WKY rats (0.66±0.05 vs 0.52±0.03nmole.mg-1 wet tissue; p<0.05). A lower activity of the transport system in the hypertensive group was evidenced by the fraction of these maximal uptake values accumulated after 2 minutes of uptake, 56% compared with 98% in the normotensive group. 45Ca release was assayed in skinned aortic strips preloaded for 30 minutes with 45Ca in the absence of K oxalate and desaturated with washing solutions containing 3nM free Ca2+. 30mM of caffeine, 5μM of norepinephrine or 10μM of IP3 resulted in greater increases in the rates of Ca2+ efflux in WKY than in SHR aortic strips. Net effluxes of Ca2+ upon stimulation with all these drugs were statistically significant only in the hypertensive group due to its slightly but consistently higher Ca2+ content. Changes in both rate of efflux and net efflux induced by 30mM of caffeine could be blocked by 0.6mM of ryanodine. The sarcoplasmic reticulum is characterized in the genetically hypertensive rats by a low transport activity of its Ca2+-ATPase, a high Ca2+ content and a Ca2+ release mechanism with low responsiveness to stimulation by caffeine, norepinephrine and IP3</description><identifier>ISSN: 0021-4868</identifier><identifier>EISSN: 1348-673X</identifier><identifier>DOI: 10.1536/ihj.36.377</identifier><identifier>PMID: 7650844</identifier><identifier>CODEN: JHEJAR</identifier><language>eng</language><publisher>Tokyo: International Heart Journal Association</publisher><subject>45Ca uptake and release ; Animals ; Aorta - metabolism ; Arterial hypertension. Arterial hypotension ; Biological and medical sciences ; Biological Transport ; Blood and lymphatic vessels ; Caffeine - pharmacology ; Calcium - metabolism ; Cardiology. Vascular system ; Experimental diseases ; Hypertension - metabolism ; Inositol Phosphates - pharmacology ; Male ; Medical sciences ; Muscle, Smooth, Vascular - metabolism ; Norepinephrine - pharmacology ; Rats ; Rats, Inbred SHR ; Rats, Inbred WKY ; Sarcoplasmic reticulum ; Sarcoplasmic Reticulum - metabolism ; Skinned smooth vascular muscle ; Spontaneously hypertensive rats</subject><ispartof>Japanese Heart Journal, 1995, Vol.36(3), pp.377-388</ispartof><rights>by International Heart Journal Association</rights><rights>1995 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-abd6ea93a94746a832c10254a77c083cbf0c314b68706224c054308520ac5cad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3648860$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7650844$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AIELLO, Ernesto A.</creatorcontrib><creatorcontrib>GRASSI, Angela O.</creatorcontrib><title>Uptake and Release of Ca2+ in Chemically Skinned Aortic Strips from Spontaneously Hypertensive (SHR) and Normotensive (WKY) Rats</title><title>Japanese Heart Journal</title><addtitle>Jpn Heart J</addtitle><description>The uptake and release of Ca2+ were studied in EGTA-skinned aortic strips from spontaneously hypertensive rats (SHR strain: SAP=191±5mmHg, n=27) and normotensive control rats (WKY strain: SAP=131±2mmHg, n=25). 45Ca uptake was measured as a function of time (0.5 to 30min.), at pCa 6.6, in the presence of 10mM of K oxalate. Skinned aortic strips of SHRs accumulated more Ca2+ after 30min of uptake than those of WKY rats (0.66±0.05 vs 0.52±0.03nmole.mg-1 wet tissue; p<0.05). A lower activity of the transport system in the hypertensive group was evidenced by the fraction of these maximal uptake values accumulated after 2 minutes of uptake, 56% compared with 98% in the normotensive group. 45Ca release was assayed in skinned aortic strips preloaded for 30 minutes with 45Ca in the absence of K oxalate and desaturated with washing solutions containing 3nM free Ca2+. 30mM of caffeine, 5μM of norepinephrine or 10μM of IP3 resulted in greater increases in the rates of Ca2+ efflux in WKY than in SHR aortic strips. Net effluxes of Ca2+ upon stimulation with all these drugs were statistically significant only in the hypertensive group due to its slightly but consistently higher Ca2+ content. Changes in both rate of efflux and net efflux induced by 30mM of caffeine could be blocked by 0.6mM of ryanodine. The sarcoplasmic reticulum is characterized in the genetically hypertensive rats by a low transport activity of its Ca2+-ATPase, a high Ca2+ content and a Ca2+ release mechanism with low responsiveness to stimulation by caffeine, norepinephrine and IP3</description><subject>45Ca uptake and release</subject><subject>Animals</subject><subject>Aorta - metabolism</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Blood and lymphatic vessels</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cardiology. Vascular system</subject><subject>Experimental diseases</subject><subject>Hypertension - metabolism</subject><subject>Inositol Phosphates - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Norepinephrine - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred SHR</subject><subject>Rats, Inbred WKY</subject><subject>Sarcoplasmic reticulum</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Skinned smooth vascular muscle</subject><subject>Spontaneously hypertensive rats</subject><issn>0021-4868</issn><issn>1348-673X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFv0zAUhy0EGmVw4Y7kA5oYKMWJHds9ThGsiIlJ7SbgFL06L9RdEgc7ReqNP33uGuXiJ_n36feePkLepmye5lx-ttvdnMs5V-oZmaVc6EQq_us5mTGWpYnQUr8kr0LYMZbKTPMzcqZkzrQQM_L_vh_gASl0FV1hgxCQupoWkH2itqPFFltroGkOdP1guw4reuX8YA1dD972gdbetXTdu26ADt0-RHB56NEP2AX7D-mH9XJ1-dT-w_nWTd8_v_--pCsYwmvyooYm4JtxnpP7r1_uimVyc3v9rbi6SUzO5JDAppIICw4LoYQEzTOTsiwXoJRhmptNzQxPxUZqxWSWCcNywZnOMwYmN1Dxc3Jx6u29-7vHMJStDQab5nR3qWLtIstlBD-eQONdCB7rsve2BX8oU1YedZdRdxlH1B3hd2PrftNiNaGj35i_H3MIUWPtoTM2TBiXQmvJIlacsF0Y4A9OORxVN3jcmC4ke9o6PkpNqdmCL7Hjj8pZnsg</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>AIELLO, Ernesto A.</creator><creator>GRASSI, Angela O.</creator><general>International Heart Journal Association</general><general>Japanese Heart Journal Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Uptake and Release of Ca2+ in Chemically Skinned Aortic Strips from Spontaneously Hypertensive (SHR) and Normotensive (WKY) Rats</title><author>AIELLO, Ernesto A. ; GRASSI, Angela O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-abd6ea93a94746a832c10254a77c083cbf0c314b68706224c054308520ac5cad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>45Ca uptake and release</topic><topic>Animals</topic><topic>Aorta - metabolism</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Blood and lymphatic vessels</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cardiology. Vascular system</topic><topic>Experimental diseases</topic><topic>Hypertension - metabolism</topic><topic>Inositol Phosphates - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Norepinephrine - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred SHR</topic><topic>Rats, Inbred WKY</topic><topic>Sarcoplasmic reticulum</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Skinned smooth vascular muscle</topic><topic>Spontaneously hypertensive rats</topic><toplevel>online_resources</toplevel><creatorcontrib>AIELLO, Ernesto A.</creatorcontrib><creatorcontrib>GRASSI, Angela O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Heart Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AIELLO, Ernesto A.</au><au>GRASSI, Angela O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uptake and Release of Ca2+ in Chemically Skinned Aortic Strips from Spontaneously Hypertensive (SHR) and Normotensive (WKY) Rats</atitle><jtitle>Japanese Heart Journal</jtitle><addtitle>Jpn Heart J</addtitle><date>1995</date><risdate>1995</risdate><volume>36</volume><issue>3</issue><spage>377</spage><epage>388</epage><pages>377-388</pages><issn>0021-4868</issn><eissn>1348-673X</eissn><coden>JHEJAR</coden><abstract>The uptake and release of Ca2+ were studied in EGTA-skinned aortic strips from spontaneously hypertensive rats (SHR strain: SAP=191±5mmHg, n=27) and normotensive control rats (WKY strain: SAP=131±2mmHg, n=25). 45Ca uptake was measured as a function of time (0.5 to 30min.), at pCa 6.6, in the presence of 10mM of K oxalate. Skinned aortic strips of SHRs accumulated more Ca2+ after 30min of uptake than those of WKY rats (0.66±0.05 vs 0.52±0.03nmole.mg-1 wet tissue; p<0.05). A lower activity of the transport system in the hypertensive group was evidenced by the fraction of these maximal uptake values accumulated after 2 minutes of uptake, 56% compared with 98% in the normotensive group. 45Ca release was assayed in skinned aortic strips preloaded for 30 minutes with 45Ca in the absence of K oxalate and desaturated with washing solutions containing 3nM free Ca2+. 30mM of caffeine, 5μM of norepinephrine or 10μM of IP3 resulted in greater increases in the rates of Ca2+ efflux in WKY than in SHR aortic strips. Net effluxes of Ca2+ upon stimulation with all these drugs were statistically significant only in the hypertensive group due to its slightly but consistently higher Ca2+ content. Changes in both rate of efflux and net efflux induced by 30mM of caffeine could be blocked by 0.6mM of ryanodine. The sarcoplasmic reticulum is characterized in the genetically hypertensive rats by a low transport activity of its Ca2+-ATPase, a high Ca2+ content and a Ca2+ release mechanism with low responsiveness to stimulation by caffeine, norepinephrine and IP3</abstract><cop>Tokyo</cop><pub>International Heart Journal Association</pub><pmid>7650844</pmid><doi>10.1536/ihj.36.377</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Japanese Heart Journal, 1995, Vol.36(3), pp.377-388 |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals |
| subjects | 45Ca uptake and release Animals Aorta - metabolism Arterial hypertension. Arterial hypotension Biological and medical sciences Biological Transport Blood and lymphatic vessels Caffeine - pharmacology Calcium - metabolism Cardiology. Vascular system Experimental diseases Hypertension - metabolism Inositol Phosphates - pharmacology Male Medical sciences Muscle, Smooth, Vascular - metabolism Norepinephrine - pharmacology Rats Rats, Inbred SHR Rats, Inbred WKY Sarcoplasmic reticulum Sarcoplasmic Reticulum - metabolism Skinned smooth vascular muscle Spontaneously hypertensive rats |
| title | Uptake and Release of Ca2+ in Chemically Skinned Aortic Strips from Spontaneously Hypertensive (SHR) and Normotensive (WKY) Rats |
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