Evidence for an inhibitory effect of the peptide galanin on dopamine release from the rat median eminence

The role of the neuropeptide galanin (GAL) in rat hypothalamus has been studied in different experimental models. Thus, the effect of GAL on potassium-induced dopamine release was analyzed in vitro, and the localization of GAL and GAL binding sites was studied with immunohistochemistry and receptor...

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Veröffentlicht in:	Neuroscience letters 1987-01, Vol.73 (1), p.21-26
Hauptverfasser:	Nordström, Öie, Melander, Tor, Hökfelt, Tomas, Bartfai, Tamas, Goldstein, Menek
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autoradiography Binding Sites Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Coexistence Dopamine - metabolism Dopamine release Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Galanin Histocytochemistry Hypothalamus - analysis In Vitro Techniques Male Median eminence Median Eminence - metabolism Neuropeptides - analysis Neuropeptides - physiology Peptides - analysis Peptides - physiology Rat Rats Rats, Inbred Strains Tyrosine 3-Monooxygenase - analysis Vertebrates: nervous system and sense organs
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Zusammenfassung:	The role of the neuropeptide galanin (GAL) in rat hypothalamus has been studied in different experimental models. Thus, the effect of GAL on potassium-induced dopamine release was analyzed in vitro, and the localization of GAL and GAL binding sites was studied with immunohistochemistry and receptor autoradiography, respectively. In the median eminence GAL and presumably dopamine were found to coexist in nerve endings and this area contained a high density of 125I-GAL binding sites. In vitro experiments revealed that GAL inhibited the release of [ 3H]dopamine in a dose-dependent manner (IC 50=7 10 nM), possibly via a presynaptic receptor.
ISSN:	0304-3940 1872-7972
DOI:	10.1016/0304-3940(87)90024-3