The first untranslated exon of the human tenascin-C gene plays a regulatory role in gene transcription

The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase t...

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Veröffentlicht in:	FEBS letters 1995-08, Vol.369 (2), p.335-339
Hauptverfasser:	Gherzi, Roberto, Ponassi, Marco, Gaggero, Barbara, Zardi, Luciano
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 Cells Animals Base Sequence Cell Adhesion Molecules, Neuronal - genetics Chloramphenicol O-Acetyltransferase - genetics Cricetinae Enhancer Enhancer Elements, Genetic - genetics Exons - genetics Extracellular Matrix Proteins - genetics Gene transcription Genes - genetics Genes, Reporter - genetics Humans Mice Molecular Sequence Data Promoter Regions, Genetic - genetics RNA, Messenger - analysis Sequence Homology, Nucleic Acid Silencer Tenascin Tenascin-C Transcription, Genetic - genetics Transfection Tumor Cells, Cultured
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Zusammenfassung:	The transcription of the human tenacin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(95)00778-8