The cDNA encoding Xenopus laevis heat-shock factor 1 (XHSF1): nucleotide and deduced amino-acid sequences, and properties of the encoded protein

The cDNA encoding Xenopus laevis heat-shock factor 1 (XHSF1): nucleotide and deduced amino-acid sequences, and properties of the encoded protein

We have isolated a cDNA encoding Xenopus laevis (Xl) heat-shock factor 1 (XHSF1). XHSF1, translated from the mRNA synthesized in vitro, will bind specifically to the X1 hsp70 promoter (hsp70). Microinjection of XHSF1 mRNA into Xl oocytes leads to synthesis of XHSF1 which accumulates in the nucleus a...

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Veröffentlicht in:Gene 1995, Vol.160 (2), p.207-211
Hauptverfasser: Stump, Donald G., Landsberger, Nicoletta, Wolffe, Alan P.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Animals
Base Sequence
Cell Nucleus - metabolism
DNA, Complementary - genetics
DNA-Binding Proteins - genetics
expression
Freshwater
Genes
Heat Shock Transcription Factors
heat-shock promoter
Molecular Sequence Data
Recombinant DNA
Recombinant Fusion Proteins - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors
Transcription, Genetic
transcriptional regulation
Xenopus laevis
Xenopus laevis - genetics
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Zusammenfassung:We have isolated a cDNA encoding Xenopus laevis (Xl) heat-shock factor 1 (XHSF1). XHSF1, translated from the mRNA synthesized in vitro, will bind specifically to the X1 hsp70 promoter (hsp70). Microinjection of XHSF1 mRNA into Xl oocytes leads to synthesis of XHSF1 which accumulates in the nucleus and selectively activates Xl phsp70p activity at 18°C.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00176-7