Oral administration of L-arginine and captopril in rats prevents chronic renal failure by nitric oxide production
Oral administration of L-arginine and captopril in rats prevents chronic renal failure by nitric oxide production. The effect of oral supplementation of L-arginine, the substrate of nitric oxide, (1.25 g/liter water) and captopril (15 mg/liter water) was studied in 5/6 nephrectomized rats for a peri...
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Veröffentlicht in: | Kidney international 1995-06, Vol.47 (6), p.1515-1521 |
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Sprache: | eng |
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Zusammenfassung: | Oral administration of L-arginine and captopril in rats prevents chronic renal failure by nitric oxide production. The effect of oral supplementation of L-arginine, the substrate of nitric oxide, (1.25 g/liter water) and captopril (15 mg/liter water) was studied in 5/6 nephrectomized rats for a period of three months. N-omega-nitro L-arginine, a nitric oxide synthase inhibitor, was given orally (70 mg/liter water) with or without L-arginine or captopril. The urinary excretion of nitrite (NO2) + nitrate (NO3), the known metabolites of nitric oxide, was taken as an index of nitric oxide production. Chronic renal failure rats were characterized by a low creatinine clearance, high FENa%, proteinuria, hypertension and a low urinary excretion of NO2 + NO3: 0.152 ± 0.06 (P < 0.001) nmol/µg creatinine compared with 0.481 ± 0.004 (P < 0.001) in normal rats and 0.479 ± 0.11 (P < 0.001) in untreated sham-operated rats. Both L-arginine and captopril were effective in the normalization of all these parameters. The combination of L-arginine and captopril had no additive effects. The nitric oxide synthase inhibitor significantly diminished the captopril beneficial effect. It is concluded that chronic renal failure in rats is a low nitric oxide production state. The supplementation of L-arginine is shown to overcome this condition. It is suggested that the beneficial effect of captopril on chronic renal failure is through a specific L-arginine—nitric oxide synthase—nitric oxide pathway. |
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ISSN: | 0085-2538 1523-1755 |
DOI: | 10.1038/ki.1995.214 |