Association of Flavin Adenine Dinucleotide with the Arabidopsis Blue Light Receptor CRY1

The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 inclu...

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Veröffentlicht in:	Science 1995-08, Vol.269 (5226), p.968-970
Hauptverfasser:	Lin, Chentao, Robertson, Dan E., Ahmad, Margaret, Raibekas, Andrei A., Jorns, Marilyn Schuman, Dutton, P. Leslie, Cashmore, Anthony R.
Format:	Artikel
Sprache:	eng
Schlagworte:	ABSORPTION Absorption spectra Analytical, structural and metabolic biochemistry ARABIDOPSIS Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins Arabidopsis thaliana Biochemistry Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES Blue light Chromophores Coding Cryptochromes Deoxyribodipyrimidine Photo-Lyase - metabolism DNA Drosophila Proteins Environmental aspects Escherichia coli Eye Proteins Feedback (Response) Flavoproteins Flavoproteins - chemistry Flavoproteins - genetics Flavoproteins - metabolism Flowers & plants Fundamental and applied biological sciences. Psychology Genes Genes, Plant Genetic aspects Holoproteins Hypocotyls Inhibition Light Monochromatic light NUCLEOTIDES Other proteins Oxidation-Reduction Photobiology Photoreceptor Cells, Invertebrate Photoreceptors Photosynthesis PLANT GROWTH Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Proteins Quinones - metabolism RECEPTORS Receptors, G-Protein-Coupled RESPONSE MODIFYING FACTORS Seedlings Sequence homology Spectrum Analysis Ultraviolet Rays VISIBLE RADIATION
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Zusammenfassung:	The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH·). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.7638620