Optimization of PCR-based diagnostics for human cytomegalovirus

Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, de...

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Veröffentlicht in:	Journal of virological methods 1995-05, Vol.53 (1), p.103-112
Hauptverfasser:	Vinogradskaya, G.R., Goryshin, I.Yu, Berlin, Yu.A., Lanzov, V.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence CMV genome Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus Infections - diagnosis Cytomegalovirus Infections - epidemiology Cytomegalovirus Infections - urine DNA, Viral - classification DNA, Viral - isolation & purification Humans Internal primer Molecular Epidemiology Molecular Sequence Data PCR Polymerase Chain Reaction - methods Sensitivity and Specificity Structural polymorphism Templates, Genetic
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Beschreibung
Zusammenfassung:	Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(95)00002-C