Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen
The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10 −4 – 10 −5) when TAW-925 was hybridized with cells tran...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1987-02, Vol.142 (3), p.805-812 |
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| container_issue | 3 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 142 |
| creator | Ichimori, Yuzo Harada, Kaori Hitotsumachi, Shinya Tsukamoto, Kyozo |
| description | The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10
−4 – 10
−5) when TAW-925 was hybridized with cells transformed with Epstein-Barr virus. Using TAW-925 as a parental cell line, we have obtained a hybridoma which stably secretes human monoclonal antibody against hepatitis B virus surface antigen. |
| doi_str_mv | 10.1016/0006-291X(87)91485-9 |
| format | Article |
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−4 – 10
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−4 – 10
−5) when TAW-925 was hybridized with cells transformed with Epstein-Barr virus. Using TAW-925 as a parental cell line, we have obtained a hybridoma which stably secretes human monoclonal antibody against hepatitis B virus surface antigen.</description><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Applied sciences</subject><subject>B-Lymphocytes - immunology</subject><subject>Cell Fusion</subject><subject>Cell Line</subject><subject>Cell Transformation, Viral</subject><subject>Exact sciences and technology</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>hepatitis B virus</subject><subject>Herpesvirus 4, Human</subject><subject>Humans</subject><subject>Hybridomas - immunology</subject><subject>Hybridomas - ultrastructure</subject><subject>Karyotyping</subject><subject>Other techniques and industries</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo6-zqP1DIQUQPrUkn3UkuC7rsqrDgRcFbyEf1dKQ7GZP0wvx7e3aGOSp1qEM9VRTPi9ArSj5QQvuPhJC-aRX99U6K94py2TXqCdpQokjTUsKfos0ZeY4uS_lNCKW8VxfogpFD0Q0ab0s1dgplnCFWnAY87m0OPs0GF3AZaohbPC6ziXhOMbkpRTNhE2uwye-x2ZoQS8Uj7EwNNRT8GT-EvBRcljwYB4_oFuIL9GwwU4GXp36Fft7d_rj52tx___Lt5tN943jHauOtlUPbS8sZA0qYZIr5zhnouO-NEC13nhFLDQHHleVCyME6ZV1rOIUW2BV6e7y7y-nPAqXqORQH02QipKVoITjrW8L_C66mmOx7sYL8CLqcSskw6F0Os8l7TYk-JKEPmvVBs5ZCPyah1br2-nR_sTP489JJ_Tp_c5qb4sw0ZBNdKGdMdK2SVK7Y9RGDVdpDgKyLCxAd-JDBVe1T-PcffwEaHqbL</recordid><startdate>19870213</startdate><enddate>19870213</enddate><creator>Ichimori, Yuzo</creator><creator>Harada, Kaori</creator><creator>Hitotsumachi, Shinya</creator><creator>Tsukamoto, Kyozo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19870213</creationdate><title>Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen</title><author>Ichimori, Yuzo ; Harada, Kaori ; Hitotsumachi, Shinya ; Tsukamoto, Kyozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-dbb8f268b433e1038393d5cae54d6a7724cd30b1a0ec49b4778fbc9bc2a41e2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Applied sciences</topic><topic>B-Lymphocytes - immunology</topic><topic>Cell Fusion</topic><topic>Cell Line</topic><topic>Cell Transformation, Viral</topic><topic>Exact sciences and technology</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>hepatitis B virus</topic><topic>Herpesvirus 4, Human</topic><topic>Humans</topic><topic>Hybridomas - immunology</topic><topic>Hybridomas - ultrastructure</topic><topic>Karyotyping</topic><topic>Other techniques and industries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichimori, Yuzo</creatorcontrib><creatorcontrib>Harada, Kaori</creatorcontrib><creatorcontrib>Hitotsumachi, Shinya</creatorcontrib><creatorcontrib>Tsukamoto, Kyozo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichimori, Yuzo</au><au>Harada, Kaori</au><au>Hitotsumachi, Shinya</au><au>Tsukamoto, Kyozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1987-02-13</date><risdate>1987</risdate><volume>142</volume><issue>3</issue><spage>805</spage><epage>812</epage><pages>805-812</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10
−4 – 10
−5) when TAW-925 was hybridized with cells transformed with Epstein-Barr virus. Using TAW-925 as a parental cell line, we have obtained a hybridoma which stably secretes human monoclonal antibody against hepatitis B virus surface antigen.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>3030301</pmid><doi>10.1016/0006-291X(87)91485-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1987-02, Vol.142 (3), p.805-812 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - biosynthesis Applied sciences B-Lymphocytes - immunology Cell Fusion Cell Line Cell Transformation, Viral Exact sciences and technology Hepatitis B Surface Antigens - immunology hepatitis B virus Herpesvirus 4, Human Humans Hybridomas - immunology Hybridomas - ultrastructure Karyotyping Other techniques and industries |
| title | Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen |
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