Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen

The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10 −4 – 10 −5) when TAW-925 was hybridized with cells tran...

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Veröffentlicht in:	Biochemical and biophysical research communications 1987-02, Vol.142 (3), p.805-812
Hauptverfasser:	Ichimori, Yuzo, Harada, Kaori, Hitotsumachi, Shinya, Tsukamoto, Kyozo
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - biosynthesis Applied sciences B-Lymphocytes - immunology Cell Fusion Cell Line Cell Transformation, Viral Exact sciences and technology Hepatitis B Surface Antigens - immunology hepatitis B virus Herpesvirus 4, Human Humans Hybridomas - immunology Hybridomas - ultrastructure Karyotyping Other techniques and industries
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Zusammenfassung:	The HAT (hypoxanthine, aminopterin, thymidine) sensitive and ouabain resistant human B lymphoblastoid cell line TAW-925 was obtained from 6-thioguanine resistant B lymphoblastoid cell line WI-L2. Hybridomas were obtained at a high frequency (10 −4 – 10 −5) when TAW-925 was hybridized with cells transformed with Epstein-Barr virus. Using TAW-925 as a parental cell line, we have obtained a hybridoma which stably secretes human monoclonal antibody against hepatitis B virus surface antigen.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(87)91485-9