Heterogeneity of β-adrenoreceptors in guinea pig alveolar type II cells

Heterogeneity of β-adrenoreceptors in guinea pig alveolar type II cells

[ 3H]Dihydroalprenolol ([ 3H]DHA) binding to guinea pig alveolar type II cell membrane revealed the presence of both high (K D= 0.38nM) and low (K D= 4.2nM) affinity β-adrenoreceptors. The low affinity site had a higher binding capacity (Bmax = 245.6 fmol/mg protein) than the high affinity site (Bma...

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Veröffentlicht in:Biochemical and biophysical research communications 1987-02, Vol.142 (3), p.898-903
Hauptverfasser: Das, Salil K., Sikpi, Matthew O., Skolnick, Phil
Format: Artikel
Sprache:eng
Schlagworte:
alveoli
Animals
Applied sciences
beta -adrenergic
Cell Membrane - metabolism
Dihydroalprenolol - metabolism
Exact sciences and technology
Guinea Pigs
Male
Other techniques and industries
Practolol - metabolism
Pulmonary Alveoli - metabolism
Receptors, Adrenergic, beta - metabolism
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Zusammenfassung:[ 3H]Dihydroalprenolol ([ 3H]DHA) binding to guinea pig alveolar type II cell membrane revealed the presence of both high (K D= 0.38nM) and low (K D= 4.2nM) affinity β-adrenoreceptors. The low affinity site had a higher binding capacity (Bmax = 245.6 fmol/mg protein) than the high affinity site (Bmax = 71.7 fmol/mg protein). Displacement of [ 3H]DHA by practolol, a selective beta 1 agent, confirmed the existence of two species of adrenoreceptors, corresponding to 21% high affinity (beta 1) and 79% low affinity (beta 2), respectively.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(87)91498-7