Tumor necrosis factor stimulates gelatinase and collagenase production by granulation tissue in culture

Tumor necrosis factor stimulates gelatinase and collagenase production by granulation tissue in culture

Tumor necrosis factor (TNF, 2.6×10 −11 – 10 −9M) caused an increase in the production of active gelatinase and latent collagenase by granulation tissue in culture. As determined by SDS-substrate polyacrylamide gel electrophoresis, granulation tissue produced mainly two species of gelatinase with mol...

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Veröffentlicht in:Biochemical and biophysical research communications 1987-02, Vol.142 (3), p.791-797
Hauptverfasser: Nakagawa, Hideo, Kitagawa, Hisashi, Aikawa, Yukihiko
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Applied sciences
Collagen - metabolism
collagenase
Culture Techniques
Electrophoresis, Polyacrylamide Gel
Enzyme Induction - drug effects
Exact sciences and technology
Gelatin - metabolism
gelatinase
Gelatinases
Glycoproteins - pharmacology
Granulation Tissue - enzymology
inflammation
Male
Microbial Collagenase - biosynthesis
Molecular Weight
Other techniques and industries
Pepsin A - biosynthesis
Rats
Rats, Inbred Strains
tumor necrosis factor
Tumor Necrosis Factor-alpha
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Zusammenfassung:Tumor necrosis factor (TNF, 2.6×10 −11 – 10 −9M) caused an increase in the production of active gelatinase and latent collagenase by granulation tissue in culture. As determined by SDS-substrate polyacrylamide gel electrophoresis, granulation tissue produced mainly two species of gelatinase with molecular weights of 64 kDa and 57 kDa, and an additional 80-kDa gelatinase was produced by TNF treatment. The results suggest that TNF may play a role in a rapid collagen turnover in the carrageenin-induced granulation tissue in rats.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(87)91483-5