A micromethod for the estimation of blood dolichol

A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 μl are sufficient to carry out the determi...

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Veröffentlicht in:	Lipids 1987-01, Vol.22 (1), p.58-60
Hauptverfasser:	Morris, George N., Pullarkat, Raju K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Age Factors Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid Diterpenes - blood Dolichol - blood Fundamental and applied biological sciences. Psychology Mice Mice, Inbred C57BL Microchemistry - methods Other biological molecules Terpenes, steroids. Hormones
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Beschreibung
Zusammenfassung:	A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 μl are sufficient to carry out the determination. Blood samples from the tail vein of inbred strains of C57 BL/6 NNia‐1 mice 3,6, 12 and 24 mo of age were examined. Blood dolichol levels decreased with age from 163.3 ng/ml at 6 mo to 110.1 ng/ml at 24 mo. The major dolichol homologs were C‐85 (10.4%), C‐90 (41.6%), C‐95 (38.0%) and C‐100 (8.1%).
ISSN:	0024-4201 1558-9307
DOI:	10.1007/BF02534876