Downregulation of Thyroid Hormone Receptor Subtype mRNA Levels by Amiodarone during Catecholamine Stress in Vitro

Amiodarone, a powerful antiarrhythmic drug, likely exerts its major effect by antagonism of thyroid hormone (T3), probably at the receptor level. T3 is known to regulate β-adrenergic receptor density in the heart but the effects of sympathomimetic drugs on thyroid hormone receptors (T3R) is not know...

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Veröffentlicht in:	Biochemical and biophysical research communications 1995-06, Vol.211 (3), p.991-996
Hauptverfasser:	Drvota, V., Bronnegard, M., Haggblad, J., Barkhem, T., Sylven, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amiodarone - pharmacology Animals Base Sequence Cells, Cultured Down-Regulation Heart - drug effects Isoproterenol - pharmacology Mice Molecular Sequence Data Myocardium - cytology Myocardium - metabolism Receptors, Thyroid Hormone - biosynthesis Receptors, Thyroid Hormone - genetics RNA, Messenger - analysis
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Beschreibung
Zusammenfassung:	Amiodarone, a powerful antiarrhythmic drug, likely exerts its major effect by antagonism of thyroid hormone (T3), probably at the receptor level. T3 is known to regulate β-adrenergic receptor density in the heart but the effects of sympathomimetic drugs on thyroid hormone receptors (T3R) is not known. The aim of this study was to investigate how amiodarone and isoproterenol affect T3R-mRNA in cultured cardiomyocytes. Confluent, isoproterenol pretreated, AT-1 cardiomyocytes were treated with isoproterenol free medium, amiodarone, T3 and amiodarone together with T3 for 48 hours. Solution hybridization for the determination of mRNA for T3R α1, α2, β1 and β2 were performed. In itself isoproterenol upregulated T3R α1, T3R β1, T3R β2 (p
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1995.1909