Evaluation of the cellmatics and direct monoclonal fluorescence antibody staining for detection of genital chlamydial infections

Three methods for Chlamydia trachomatis detection were compared: (1) the Cellmatics®(Difco Laboratories, Detroit, MI), a commercially available tissue culture system which contains a rat cell line; (2) a direct fluorescent Chlamydia antibody (DFA) (Microtrak, Syva Corp., Palo Alto, California) stain...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 1987-02, Vol.6 (2), p.139-143
Hauptverfasser:	Peterson, Ellena M., Alexander, Richard, Aarnaes, Sandra L., King, Susan, Greenwood, J.R., de la Maza, Luis M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Cell Line Cells, Cultured Cervix Uteri - microbiology Chlamydia Infections - diagnosis Chlamydia trachomatis - isolation & purification Female Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genital Diseases, Female - diagnosis Genital Diseases, Male - diagnosis Humans Male Microbiology Urethra - microbiology
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Zusammenfassung:	Three methods for Chlamydia trachomatis detection were compared: (1) the Cellmatics®(Difco Laboratories, Detroit, MI), a commercially available tissue culture system which contains a rat cell line; (2) a direct fluorescent Chlamydia antibody (DFA) (Microtrak, Syva Corp., Palo Alto, California) stain; and (3) a standard tissue culture isolation method employing McCoy cells. Of the 121 specimens in the study, 20 were positive by the standard cell culture. All 20 of these specimens were also positive by the Cellmatics® but only 10 were positive by DFA.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/0732-8893(87)90098-8