Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide

A rabbit antiserum to the mouse CD8 antigen ( Lyt- 2 3 ) was obtained through the use of a synthetic peptide corresponding to the N-terminal segment of the 37 kDa subunit of the CD8 molecular complex. This anti-peptide antiserum detected specifically the membrane antigen from detergent extracts of s...

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Veröffentlicht in:	Journal of immunological methods 1987-01, Vol.96 (1), p.97-105
Hauptverfasser:	Brunati, S., Corradin, G., Bron, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Antibody Specificity Antigens, Differentiation, T-Lymphocyte Antigens, Surface - immunology Antiserum Biological and medical sciences Fundamental and applied biological sciences. Psychology Fundamental immunology Immune Sera Immunization Immunoelectrophoresis Lymphocyte membrane Lyt-2 antigen Membrane Proteins - immunology Mice Molecular immunology Peptide Fragments - chemical synthesis Peptide Fragments - immunology rabbit Rabbits Synthetic peptide T-Lymphocytes - immunology Techniques
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Zusammenfassung:	A rabbit antiserum to the mouse CD8 antigen ( Lyt- 2 3 ) was obtained through the use of a synthetic peptide corresponding to the N-terminal segment of the 37 kDa subunit of the CD8 molecular complex. This anti-peptide antiserum detected specifically the membrane antigen from detergent extracts of surface-labeled mouse thymocytes. The efficiency of the immunoprecipitation increased significantly upon treatment of the cell lysates with 0.1% SDS at 100°C before immunopurification. Finally both the 37 kDa and the 32 kDa polypeptides expressing the Lyt-2 antigen were revealed by immunoblotting.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(87)90372-3