Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors
We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times betw...
Gespeichert in:
| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1987-01, Vol.35 (1), p.87-92 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 92 |
|---|---|
| container_issue | 1 |
| container_start_page | 87 |
| container_title | The journal of histochemistry and cytochemistry |
| container_volume | 35 |
| creator | Stumpf, WE Morin, JK Ennis, BW Zielinski, JE Hochberg, RB |
| description | We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution. |
| doi_str_mv | 10.1177/35.1.3794310 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77369753</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_35.1.3794310</sage_id><sourcerecordid>77369753</sourcerecordid><originalsourceid>FETCH-LOGICAL-c407t-19ed77d5767608b9537a22349270d5a598173c9a8ec27fa633886b517ea739873</originalsourceid><addsrcrecordid>eNqNkEFr3DAQRkVpSTdpb70WdCiFQr2VLMljH0No00Agl-RUipi15LWCduVKNsse-t-r3TXJKdDTMDOPx8xHyAfOlpwDfBNqyZcCGik4e0UWXCleKCbla7JgrCyLPJBvyXlKj4xxKVV9Rs5mfEH-PozOu3FPQ0d_8YqiH3oseKluflMXTLBpjGhc8LQLkeI0hmO7jjj0--Ns7C1N42SOitZ6P3nM5NbQaNcubNFT47LFraYxtwcq2tYO2ZTekTcd-mTfz_WCPPz4fn_1s7i9u765urwtWslgLHhjDYBRUEHF6lWjBGBZCtmUwIxC1dQcRNtgbdsSOqyEqOtqpThYBNHUIC7I55N3iOHPlH_SG5cOt-LWhilpAFE1oEQGv57ANoaUou30EN0G415zpg9pa6E013N8Gf84e6fVxpon-Hn_ad5jatF3EbetS09YnTlg8j8wYNXhiy8nLOHa6scwxRxueumyWdm7db9z0eq0Qe_znVzvdrsjm4P5B34lrDo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77369753</pqid></control><display><type>article</type><title>Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors</title><source>Access via SAGE</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Stumpf, WE ; Morin, JK ; Ennis, BW ; Zielinski, JE ; Hochberg, RB</creator><creatorcontrib>Stumpf, WE ; Morin, JK ; Ennis, BW ; Zielinski, JE ; Hochberg, RB</creatorcontrib><description>We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1177/35.1.3794310</identifier><identifier>PMID: 3794310</identifier><identifier>CODEN: JHCYAS</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Animals ; Applied sciences ; Autoradiography ; Biological and medical sciences ; Brain - cytology ; Brain Chemistry ; Estradiol - analogs & derivatives ; Evaluation Studies as Topic ; Exact sciences and technology ; Female ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Iodine Radioisotopes ; Mammalian female genital system ; Other techniques and industries ; Pituitary Gland - analysis ; Pituitary Gland - cytology ; Rats ; Rats, Inbred Strains ; Receptors, Estrogen - analysis ; Uterus - analysis ; Uterus - cytology ; Vertebrates: reproduction</subject><ispartof>The journal of histochemistry and cytochemistry, 1987-01, Vol.35 (1), p.87-92</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-19ed77d5767608b9537a22349270d5a598173c9a8ec27fa633886b517ea739873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/35.1.3794310$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/35.1.3794310$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,4024,21819,27923,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8377067$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8379704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3794310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stumpf, WE</creatorcontrib><creatorcontrib>Morin, JK</creatorcontrib><creatorcontrib>Ennis, BW</creatorcontrib><creatorcontrib>Zielinski, JE</creatorcontrib><creatorcontrib>Hochberg, RB</creatorcontrib><title>Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Brain - cytology</subject><subject>Brain Chemistry</subject><subject>Estradiol - analogs & derivatives</subject><subject>Evaluation Studies as Topic</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Iodine Radioisotopes</subject><subject>Mammalian female genital system</subject><subject>Other techniques and industries</subject><subject>Pituitary Gland - analysis</subject><subject>Pituitary Gland - cytology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Estrogen - analysis</subject><subject>Uterus - analysis</subject><subject>Uterus - cytology</subject><subject>Vertebrates: reproduction</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFr3DAQRkVpSTdpb70WdCiFQr2VLMljH0No00Agl-RUipi15LWCduVKNsse-t-r3TXJKdDTMDOPx8xHyAfOlpwDfBNqyZcCGik4e0UWXCleKCbla7JgrCyLPJBvyXlKj4xxKVV9Rs5mfEH-PozOu3FPQ0d_8YqiH3oseKluflMXTLBpjGhc8LQLkeI0hmO7jjj0--Ns7C1N42SOitZ6P3nM5NbQaNcubNFT47LFraYxtwcq2tYO2ZTekTcd-mTfz_WCPPz4fn_1s7i9u765urwtWslgLHhjDYBRUEHF6lWjBGBZCtmUwIxC1dQcRNtgbdsSOqyEqOtqpThYBNHUIC7I55N3iOHPlH_SG5cOt-LWhilpAFE1oEQGv57ANoaUou30EN0G415zpg9pa6E013N8Gf84e6fVxpon-Hn_ad5jatF3EbetS09YnTlg8j8wYNXhiy8nLOHa6scwxRxueumyWdm7db9z0eq0Qe_znVzvdrsjm4P5B34lrDo</recordid><startdate>19870101</startdate><enddate>19870101</enddate><creator>Stumpf, WE</creator><creator>Morin, JK</creator><creator>Ennis, BW</creator><creator>Zielinski, JE</creator><creator>Hochberg, RB</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870101</creationdate><title>Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors</title><author>Stumpf, WE ; Morin, JK ; Ennis, BW ; Zielinski, JE ; Hochberg, RB</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-19ed77d5767608b9537a22349270d5a598173c9a8ec27fa633886b517ea739873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Brain - cytology</topic><topic>Brain Chemistry</topic><topic>Estradiol - analogs & derivatives</topic><topic>Evaluation Studies as Topic</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Iodine Radioisotopes</topic><topic>Mammalian female genital system</topic><topic>Other techniques and industries</topic><topic>Pituitary Gland - analysis</topic><topic>Pituitary Gland - cytology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Estrogen - analysis</topic><topic>Uterus - analysis</topic><topic>Uterus - cytology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stumpf, WE</creatorcontrib><creatorcontrib>Morin, JK</creatorcontrib><creatorcontrib>Ennis, BW</creatorcontrib><creatorcontrib>Zielinski, JE</creatorcontrib><creatorcontrib>Hochberg, RB</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stumpf, WE</au><au>Morin, JK</au><au>Ennis, BW</au><au>Zielinski, JE</au><au>Hochberg, RB</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1987-01-01</date><risdate>1987</risdate><volume>35</volume><issue>1</issue><spage>87</spage><epage>92</epage><pages>87-92</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>3794310</pmid><doi>10.1177/35.1.3794310</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1554 |
| ispartof | The journal of histochemistry and cytochemistry, 1987-01, Vol.35 (1), p.87-92 |
| issn | 0022-1554 1551-5044 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77369753 |
| source | Access via SAGE; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Applied sciences Autoradiography Biological and medical sciences Brain - cytology Brain Chemistry Estradiol - analogs & derivatives Evaluation Studies as Topic Exact sciences and technology Female Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Iodine Radioisotopes Mammalian female genital system Other techniques and industries Pituitary Gland - analysis Pituitary Gland - cytology Rats Rats, Inbred Strains Receptors, Estrogen - analysis Uterus - analysis Uterus - cytology Vertebrates: reproduction |
| title | Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T13%3A32%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Utility%20of%20%5B16%20alpha-125I%5D%20iodoestradiol%20for%20autoradiography%20for%20the%20study%20of%20cellular%20and%20regional%20distribution%20of%20receptors&rft.jtitle=The%20journal%20of%20histochemistry%20and%20cytochemistry&rft.au=Stumpf,%20WE&rft.date=1987-01-01&rft.volume=35&rft.issue=1&rft.spage=87&rft.epage=92&rft.pages=87-92&rft.issn=0022-1554&rft.eissn=1551-5044&rft.coden=JHCYAS&rft_id=info:doi/10.1177/35.1.3794310&rft_dat=%3Cproquest_cross%3E77369753%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77369753&rft_id=info:pmid/3794310&rft_sage_id=10.1177_35.1.3794310&rfr_iscdi=true |