Utility of [16 alpha-125I] iodoestradiol for autoradiography for the study of cellular and regional distribution of receptors

We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times betw...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 1987-01, Vol.35 (1), p.87-92
Hauptverfasser:	Stumpf, WE, Morin, JK, Ennis, BW, Zielinski, JE, Hochberg, RB
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Autoradiography Biological and medical sciences Brain - cytology Brain Chemistry Estradiol - analogs & derivatives Evaluation Studies as Topic Exact sciences and technology Female Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Iodine Radioisotopes Mammalian female genital system Other techniques and industries Pituitary Gland - analysis Pituitary Gland - cytology Rats Rats, Inbred Strains Receptors, Estrogen - analysis Uterus - analysis Uterus - cytology Vertebrates: reproduction
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Zusammenfassung:	We demonstrate the utility of [16 alpha-125I]iodoestradiol for thaw-mount autoradiography with 2 micron and 4 micron thick sections of rat and mouse uterus, pituitary, and brain after in vivo administration. Under the conditions of the experiments, short-term autoradiography with exposure times between 3 and 14 days provides optimal cellular resolution, whereas long-term autoradiography with 1-2 months of exposure may be used to obtain topographic-regional surveys of distribution.
ISSN:	0022-1554 1551-5044
DOI:	10.1177/35.1.3794310