Microdomain formation in phosphatidylethanolamine bilayers detected by 2H-NMR
In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet [Yoshikawa et al., (1988) Biochim. Biophys. Acta 944, 321–328]. This suggests the presence of two backbone conformat...
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container_title | Chemistry and physics of lipids |
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creator | Shin, Kyong-hwa Nagamori, Toshiaki Kimura, Yasuhiro Tomoi, Masao Fujiwara, Toshimichi Akutsu, Hideo |
description | In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet [Yoshikawa et al., (1988) Biochim. Biophys. Acta 944, 321–328]. This suggests the presence of two backbone conformations, the exchange between which is slow on an NMR time-scale. The origin of the two conformations has been investigated in this work using saturated 1,2-diacyl-
sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids. |
doi_str_mv | 10.1016/0009-3084(94)02435-8 |
format | Article |
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sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids.</description><identifier>ISSN: 0009-3084</identifier><identifier>EISSN: 1873-2941</identifier><identifier>DOI: 10.1016/0009-3084(94)02435-8</identifier><identifier>PMID: 7788799</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Calorimetry, Differential Scanning ; Deuterium NMR ; Escherichia coli - chemistry ; Lipid bilayer ; Lipid Bilayers - chemistry ; Magnetic Resonance Spectroscopy ; Microdomain ; Molecular Conformation ; Phosp hatidylethanolamine ; Phosphatidylethanolamines - chemistry ; Quadrupole splitting ; Specific deuteration ; Temperature</subject><ispartof>Chemistry and physics of lipids, 1995-05, Vol.76 (1), p.55-62</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1539-904e9714958de133033dad838e8be951ce1ba22350e13fe65142f50b766064cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009308494024358$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7788799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Kyong-hwa</creatorcontrib><creatorcontrib>Nagamori, Toshiaki</creatorcontrib><creatorcontrib>Kimura, Yasuhiro</creatorcontrib><creatorcontrib>Tomoi, Masao</creatorcontrib><creatorcontrib>Fujiwara, Toshimichi</creatorcontrib><creatorcontrib>Akutsu, Hideo</creatorcontrib><title>Microdomain formation in phosphatidylethanolamine bilayers detected by 2H-NMR</title><title>Chemistry and physics of lipids</title><addtitle>Chem Phys Lipids</addtitle><description>In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet [Yoshikawa et al., (1988) Biochim. Biophys. Acta 944, 321–328]. This suggests the presence of two backbone conformations, the exchange between which is slow on an NMR time-scale. The origin of the two conformations has been investigated in this work using saturated 1,2-diacyl-
sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids.</description><subject>Calorimetry, Differential Scanning</subject><subject>Deuterium NMR</subject><subject>Escherichia coli - chemistry</subject><subject>Lipid bilayer</subject><subject>Lipid Bilayers - chemistry</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Microdomain</subject><subject>Molecular Conformation</subject><subject>Phosp hatidylethanolamine</subject><subject>Phosphatidylethanolamines - chemistry</subject><subject>Quadrupole splitting</subject><subject>Specific deuteration</subject><subject>Temperature</subject><issn>0009-3084</issn><issn>1873-2941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLAzEQhYMotVb_gcKeRA-rySbZTS6CFLVCqyB6DtlklkZ2NzXZCv33prZ49DQ83ps3zIfQOcE3BJPyFmMsc4oFu5LsGheM8lwcoDERFc0LycghGv9FjtFJjJ9JYs7JCI2qSohKyjFaLJwJ3vpOuz5rfOj04HyfJbFa-rhaJmk3LQxL3ftWd66HrHat3kCImYUBzAA2qzdZMctfFm-n6KjRbYSz_Zygj8eH9-ksn78-PU_v57khnMpcYgayIkxyYYFQiim12goqQNQgOTFAal0UlOPkNlBywoqG47oqS1wyY-gEXe56V8F_rSEOqnPRQNvqHvw6qqqiLLXiFGS7YHoyxgCNWgXX6bBRBKstRbVFpLaIlGTql6ISae1i37-uO7B_S3tsyb_b-ZCe_HYQVDQOegPWhYREWe_-P_AD2JqALg</recordid><startdate>19950522</startdate><enddate>19950522</enddate><creator>Shin, Kyong-hwa</creator><creator>Nagamori, Toshiaki</creator><creator>Kimura, Yasuhiro</creator><creator>Tomoi, Masao</creator><creator>Fujiwara, Toshimichi</creator><creator>Akutsu, Hideo</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950522</creationdate><title>Microdomain formation in phosphatidylethanolamine bilayers detected by 2H-NMR</title><author>Shin, Kyong-hwa ; Nagamori, Toshiaki ; Kimura, Yasuhiro ; Tomoi, Masao ; Fujiwara, Toshimichi ; Akutsu, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1539-904e9714958de133033dad838e8be951ce1ba22350e13fe65142f50b766064cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Calorimetry, Differential Scanning</topic><topic>Deuterium NMR</topic><topic>Escherichia coli - chemistry</topic><topic>Lipid bilayer</topic><topic>Lipid Bilayers - chemistry</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Microdomain</topic><topic>Molecular Conformation</topic><topic>Phosp hatidylethanolamine</topic><topic>Phosphatidylethanolamines - chemistry</topic><topic>Quadrupole splitting</topic><topic>Specific deuteration</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Kyong-hwa</creatorcontrib><creatorcontrib>Nagamori, Toshiaki</creatorcontrib><creatorcontrib>Kimura, Yasuhiro</creatorcontrib><creatorcontrib>Tomoi, Masao</creatorcontrib><creatorcontrib>Fujiwara, Toshimichi</creatorcontrib><creatorcontrib>Akutsu, Hideo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry and physics of lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Kyong-hwa</au><au>Nagamori, Toshiaki</au><au>Kimura, Yasuhiro</au><au>Tomoi, Masao</au><au>Fujiwara, Toshimichi</au><au>Akutsu, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microdomain formation in phosphatidylethanolamine bilayers detected by 2H-NMR</atitle><jtitle>Chemistry and physics of lipids</jtitle><addtitle>Chem Phys Lipids</addtitle><date>1995-05-22</date><risdate>1995</risdate><volume>76</volume><issue>1</issue><spage>55</spage><epage>62</epage><pages>55-62</pages><issn>0009-3084</issn><eissn>1873-2941</eissn><abstract>In deuterium NMR spectra of phosphatidylethanolamine bilayers with an extremely high content of saturated fatty acids, each C1 deuteron of the glycerol backbone gave rise to a doublet [Yoshikawa et al., (1988) Biochim. Biophys. Acta 944, 321–328]. This suggests the presence of two backbone conformations, the exchange between which is slow on an NMR time-scale. The origin of the two conformations has been investigated in this work using saturated 1,2-diacyl-
sn-glycero-3-phosphoethanolamine specifically deuterated in the glycerol backbone. The results showed that the two conformations originate from different domains, which have different fatty acid compositions. The differential scanning calorimetry of the bilayers suggested that the size of the domain is not large enough to show an independent phase transition. Thus, the formation of microdomains in the phosphatidylethanolamine bilayers has been concluded. Conformational difference in different domains was shown to be restricted to the C1 position of the glycerol backbone. The microdomains of phosphatidylethanolamine were retained even in the presence of other phospholipids.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>7788799</pmid><doi>10.1016/0009-3084(94)02435-8</doi><tpages>8</tpages></addata></record> |
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subjects | Calorimetry, Differential Scanning Deuterium NMR Escherichia coli - chemistry Lipid bilayer Lipid Bilayers - chemistry Magnetic Resonance Spectroscopy Microdomain Molecular Conformation Phosp hatidylethanolamine Phosphatidylethanolamines - chemistry Quadrupole splitting Specific deuteration Temperature |
title | Microdomain formation in phosphatidylethanolamine bilayers detected by 2H-NMR |
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