Initial culture behaviour of rat blastocysts on selected feeder cell lines
To increase our understanding of rat embryos in culture and to attempt the isolation of blastocyst‐derived cell lines, we examinated the initial growth behaviour of rat blastocysts from four strains of rat on four different feeder cell layers. The feeders used were a continuous cell line of murine e...
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Veröffentlicht in: | Molecular reproduction and development 1995-03, Vol.40 (3), p.311-324 |
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description | To increase our understanding of rat embryos in culture and to attempt the isolation of blastocyst‐derived cell lines, we examinated the initial growth behaviour of rat blastocysts from four strains of rat on four different feeder cell layers. The feeders used were a continuous cell line of murine embryonic fibroblasts (STO), primary mouse (MEF) or primary rat (REF) embryonic fibroblasts, and a continuous cell line of rat uterine epithelial cells (RUCs). A medium that gave optimum plating efficiencies for murine ES cells was used in the rat embryo culture. Each culture system allowed hatching and attachment of the blastocysts, that is, the behaviour was similar on each feeder and each strain for the first 2 days in culture. Subsequently, there was a rapid differentiation of the Inner Cell Mass (ICM) cells on fibroblastic feeder cell layers (STO > MEF > REF), and this was generally complete after 3–6 days in primary culture. On RUCs, the ICM was found to increase in size without differentiation up to and including day 4 and in some cases longer. Embryo‐derived cells were obtained by disaggregating and passaging ICMs on REF and RUC feeders. Rounded, refractile, and epithelial‐like cells were isolated on REF and colonies of ES‐like cells on the RUCs. The ES‐like cells were positive for expression of alkaline phosphatase and stage‐specific embryonic‐antigen 1. This is an important first step towards the derivation and culture of pluripotent ES cells from the rat. © 1995 Wiley‐Liss, Inc. |
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The feeders used were a continuous cell line of murine embryonic fibroblasts (STO), primary mouse (MEF) or primary rat (REF) embryonic fibroblasts, and a continuous cell line of rat uterine epithelial cells (RUCs). A medium that gave optimum plating efficiencies for murine ES cells was used in the rat embryo culture. Each culture system allowed hatching and attachment of the blastocysts, that is, the behaviour was similar on each feeder and each strain for the first 2 days in culture. Subsequently, there was a rapid differentiation of the Inner Cell Mass (ICM) cells on fibroblastic feeder cell layers (STO > MEF > REF), and this was generally complete after 3–6 days in primary culture. On RUCs, the ICM was found to increase in size without differentiation up to and including day 4 and in some cases longer. Embryo‐derived cells were obtained by disaggregating and passaging ICMs on REF and RUC feeders. Rounded, refractile, and epithelial‐like cells were isolated on REF and colonies of ES‐like cells on the RUCs. The ES‐like cells were positive for expression of alkaline phosphatase and stage‐specific embryonic‐antigen 1. This is an important first step towards the derivation and culture of pluripotent ES cells from the rat. © 1995 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.1080400307</identifier><identifier>PMID: 7772341</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Biological and medical sciences ; Blastocyst - cytology ; Blastocyst - enzymology ; Blastocyst - immunology ; Cell Differentiation ; Cell Line ; Culture Media ; Culture Techniques - methods ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryo, Mammalian - cytology ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic and Fetal Development ; Epithelial Cells ; ES-like cells ; Female ; Fibroblasts - cytology ; Fundamental and applied biological sciences. Psychology ; ICM ; Immunocytochemistry ; Lewis X Antigen - metabolism ; Mice ; Rat embryos ; Rats ; Rats, Inbred F344 ; Rats, Inbred WKY ; Rats, Sprague-Dawley ; Rats, Wistar ; Stem Cells - cytology ; Uterine cells ; Uterus - cytology</subject><ispartof>Molecular reproduction and development, 1995-03, Vol.40 (3), p.311-324</ispartof><rights>Copyright © 1995 Wiley‐Liss, Inc.</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4077-1505e865213a49a1218725c5cfb1b6b7e5a5b24c24924e011831807811a375413</citedby><cites>FETCH-LOGICAL-c4077-1505e865213a49a1218725c5cfb1b6b7e5a5b24c24924e011831807811a375413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.1080400307$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.1080400307$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3449324$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7772341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ouhibi, Nadia</creatorcontrib><creatorcontrib>Sullivan, Neil F.</creatorcontrib><creatorcontrib>English, Jennifer</creatorcontrib><creatorcontrib>Colledge, William H.</creatorcontrib><creatorcontrib>Evans, Martin J.</creatorcontrib><creatorcontrib>Clarke, Neil J.</creatorcontrib><title>Initial culture behaviour of rat blastocysts on selected feeder cell lines</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>To increase our understanding of rat embryos in culture and to attempt the isolation of blastocyst‐derived cell lines, we examinated the initial growth behaviour of rat blastocysts from four strains of rat on four different feeder cell layers. The feeders used were a continuous cell line of murine embryonic fibroblasts (STO), primary mouse (MEF) or primary rat (REF) embryonic fibroblasts, and a continuous cell line of rat uterine epithelial cells (RUCs). A medium that gave optimum plating efficiencies for murine ES cells was used in the rat embryo culture. Each culture system allowed hatching and attachment of the blastocysts, that is, the behaviour was similar on each feeder and each strain for the first 2 days in culture. Subsequently, there was a rapid differentiation of the Inner Cell Mass (ICM) cells on fibroblastic feeder cell layers (STO > MEF > REF), and this was generally complete after 3–6 days in primary culture. On RUCs, the ICM was found to increase in size without differentiation up to and including day 4 and in some cases longer. Embryo‐derived cells were obtained by disaggregating and passaging ICMs on REF and RUC feeders. Rounded, refractile, and epithelial‐like cells were isolated on REF and colonies of ES‐like cells on the RUCs. The ES‐like cells were positive for expression of alkaline phosphatase and stage‐specific embryonic‐antigen 1. This is an important first step towards the derivation and culture of pluripotent ES cells from the rat. © 1995 Wiley‐Liss, Inc.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - cytology</subject><subject>Blastocyst - enzymology</subject><subject>Blastocyst - immunology</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Culture Media</subject><subject>Culture Techniques - methods</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic and Fetal Development</subject><subject>Epithelial Cells</subject><subject>ES-like cells</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ICM</subject><subject>Immunocytochemistry</subject><subject>Lewis X Antigen - metabolism</subject><subject>Mice</subject><subject>Rat embryos</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Rats, Inbred WKY</subject><subject>Rats, Sprague-Dawley</subject><subject>Rats, Wistar</subject><subject>Stem Cells - cytology</subject><subject>Uterine cells</subject><subject>Uterus - cytology</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElP5DAQhS3EiG24ckPyAc0tTHlrO0eWoQGxSGgQ3CzHXREGdwJ2AvS_J61uNZrTnKqk-l7Vq0fIHoNDBsB_T9NkaAxIAAF6jWwxKE3BdanW572EQir-uEm2c34GgLI0sEE2tNZcSLZFLi-a0AUXqe9j1yekFT6599D2ibY1Ta6jVXS5a_0sd5m2Dc0Y0Xc4oTXiBBP1GCONocH8k_yoXcy4u6w75P7sz9-T8-LqdnxxcnRVeAlaF0yBQjNSnAknS8c4M5orr3xdsWpUaVROVVx6LksuERgzghnQhjEntJJM7JBfi72vqX3rMXd2GvLchmuw7bPVWnDOzWgADxegT23OCWv7msLUpZllYOfh2SE8-x3eINhfbu6rKU5W-DKtYX6wnLvsXayTa3zIK0xIWQouB6xcYB8h4uw_R-313ek_FoqFNuQOP1dal17sSA__24ebseXX58eXZiztnfgCX4uU0Q</recordid><startdate>199503</startdate><enddate>199503</enddate><creator>Ouhibi, Nadia</creator><creator>Sullivan, Neil F.</creator><creator>English, Jennifer</creator><creator>Colledge, William H.</creator><creator>Evans, Martin J.</creator><creator>Clarke, Neil J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199503</creationdate><title>Initial culture behaviour of rat blastocysts on selected feeder cell lines</title><author>Ouhibi, Nadia ; Sullivan, Neil F. ; English, Jennifer ; Colledge, William H. ; Evans, Martin J. ; Clarke, Neil J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4077-1505e865213a49a1218725c5cfb1b6b7e5a5b24c24924e011831807811a375413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - cytology</topic><topic>Blastocyst - enzymology</topic><topic>Blastocyst - immunology</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Culture Media</topic><topic>Culture Techniques - methods</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic and Fetal Development</topic><topic>Epithelial Cells</topic><topic>ES-like cells</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ICM</topic><topic>Immunocytochemistry</topic><topic>Lewis X Antigen - metabolism</topic><topic>Mice</topic><topic>Rat embryos</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Rats, Inbred WKY</topic><topic>Rats, Sprague-Dawley</topic><topic>Rats, Wistar</topic><topic>Stem Cells - cytology</topic><topic>Uterine cells</topic><topic>Uterus - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ouhibi, Nadia</creatorcontrib><creatorcontrib>Sullivan, Neil F.</creatorcontrib><creatorcontrib>English, Jennifer</creatorcontrib><creatorcontrib>Colledge, William H.</creatorcontrib><creatorcontrib>Evans, Martin J.</creatorcontrib><creatorcontrib>Clarke, Neil J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ouhibi, Nadia</au><au>Sullivan, Neil F.</au><au>English, Jennifer</au><au>Colledge, William H.</au><au>Evans, Martin J.</au><au>Clarke, Neil J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Initial culture behaviour of rat blastocysts on selected feeder cell lines</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1995-03</date><risdate>1995</risdate><volume>40</volume><issue>3</issue><spage>311</spage><epage>324</epage><pages>311-324</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>To increase our understanding of rat embryos in culture and to attempt the isolation of blastocyst‐derived cell lines, we examinated the initial growth behaviour of rat blastocysts from four strains of rat on four different feeder cell layers. The feeders used were a continuous cell line of murine embryonic fibroblasts (STO), primary mouse (MEF) or primary rat (REF) embryonic fibroblasts, and a continuous cell line of rat uterine epithelial cells (RUCs). A medium that gave optimum plating efficiencies for murine ES cells was used in the rat embryo culture. Each culture system allowed hatching and attachment of the blastocysts, that is, the behaviour was similar on each feeder and each strain for the first 2 days in culture. Subsequently, there was a rapid differentiation of the Inner Cell Mass (ICM) cells on fibroblastic feeder cell layers (STO > MEF > REF), and this was generally complete after 3–6 days in primary culture. On RUCs, the ICM was found to increase in size without differentiation up to and including day 4 and in some cases longer. Embryo‐derived cells were obtained by disaggregating and passaging ICMs on REF and RUC feeders. Rounded, refractile, and epithelial‐like cells were isolated on REF and colonies of ES‐like cells on the RUCs. The ES‐like cells were positive for expression of alkaline phosphatase and stage‐specific embryonic‐antigen 1. This is an important first step towards the derivation and culture of pluripotent ES cells from the rat. © 1995 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>7772341</pmid><doi>10.1002/mrd.1080400307</doi><tpages>14</tpages></addata></record> |
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subjects | Alkaline Phosphatase - metabolism Animals Biological and medical sciences Blastocyst - cytology Blastocyst - enzymology Blastocyst - immunology Cell Differentiation Cell Line Culture Media Culture Techniques - methods Early stages. Segmentation. Gastrulation. Neurulation Embryo, Mammalian - cytology Embryology: invertebrates and vertebrates. Teratology Embryonic and Fetal Development Epithelial Cells ES-like cells Female Fibroblasts - cytology Fundamental and applied biological sciences. Psychology ICM Immunocytochemistry Lewis X Antigen - metabolism Mice Rat embryos Rats Rats, Inbred F344 Rats, Inbred WKY Rats, Sprague-Dawley Rats, Wistar Stem Cells - cytology Uterine cells Uterus - cytology |
title | Initial culture behaviour of rat blastocysts on selected feeder cell lines |
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