A shuttle vector system for Brevibacterium lactofermentum

We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteri...

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Veröffentlicht in:	Gene 1986, Vol.47 (2-3), p.301-306
Hauptverfasser:	YEH, P, OREGLIA, J, PREVOTS, F, SICARD, A. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Brevibacterium - drug effects Brevibacterium - genetics Brevibacterium lactofermentum cloning vectors Corynebacterium - genetics Corynebacterium glutamicum DNA, Bacterial - genetics Erythromycin - pharmacology Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology gene transfer Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Plasmids R Factors Transformation, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Beschreibung
Zusammenfassung:	We have constructed a shuttle vector that replicates in Escherichia coli, Corynebacterium glutamicum and Brevibacterium lactofermentum, by fusion of a 4.4-kb cryptic plasmid isolated from B. lactofermentum and a derivative of pBR322. Resistance to erythromycin which is expressed in all three bacteria has been a useful selective marker. The frequency of homospecific transformation was 1.5 X 10(5) transformants/micrograms of hybrid plasmid DNA.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(86)90074-0