Specific Detection of Campylobacter jejuni by means of Polymerase Chain Reaction in Chicken Litter

A routine detection assay of Campylobacter jejuni was developed with the polymerase chain reaction (PCR). A pair of oliginucleotide primers from the flaA of C. jejuni were used in detection by PCR. Although the primer pair specifically detected C. jejuni strains, they did not detect strains of C. co...

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Veröffentlicht in:	Journal of Veterinary Medical Science 1995/02/15, Vol.57(1), pp.125-127
Hauptverfasser:	ITOH, Ryu, SAITOH, Shioko, YATSUYANAGI, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Campylobacter jejuni Campylobacter jejuni - isolation & purification chicken litter Chickens - microbiology Housing, Animal Manure - microbiology Molecular Sequence Data Polymerase chain reaction Polymerase Chain Reaction - veterinary
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Zusammenfassung:	A routine detection assay of Campylobacter jejuni was developed with the polymerase chain reaction (PCR). A pair of oliginucleotide primers from the flaA of C. jejuni were used in detection by PCR. Although the primer pair specifically detected C. jejuni strains, they did not detect strains of C. coli, C. fetus, Salmonella spp. or Escherichia coli. In litter inoculated with C. jejuni, specific detection of C. jejuni was obtained by PCR with this primer pair.
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.57.125