Caffeine demethylation measured by breath analysis in experimental liver injury in the rat
To assess the effects of experimental liver injury on caffeine metabolism, 1 μCi/kg b.w. of [3-methyl 14C]-caffeine (together with 5 mg/kg b.w. of the cold compound) was injected i.p. to four different experimental groups and respective controls of unanesthetized male Sprague-Dawley rats. Exhaled 14...
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| Veröffentlicht in: | Journal of hepatology 1995, Vol.22 (1), p.82-87 |
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| creator | Schaad, Heinz J. Renner, Eberhard L. Wietholtz, Hubertus Arnaud, Maurice J. Preisig, Rudolf |
| description | To assess the effects of experimental liver injury on caffeine metabolism, 1 μCi/kg b.w. of [3-methyl
14C]-caffeine (together with 5 mg/kg b.w. of the cold compound) was injected i.p. to four different experimental groups and respective controls of unanesthetized male Sprague-Dawley rats. Exhaled
14CO
2 was completely collected during 4 h and peak exhalation rate and fraction of dose recovered were calculated.
1
3
hepatectomy affected
14CO
2 exhalation to a limited extent, decreasing solely peak exhalation rate (
p |
| doi_str_mv | 10.1016/0168-8278(95)80264-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77277289</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0168827895802649</els_id><sourcerecordid>77277289</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-6b027770844faf2d47988f7088e0f87d4fdf51a84ad8eb3c1ba2dcf39a66cf313</originalsourceid><addsrcrecordid>eNp9kE1r3DAQhkVoSTdp_kEKOpSSHNxKtmzJl0BZmqQQ6KW99CLG0ohV8MdWkkP97ytnlz32IAZpnnkZPYRcc_aZM958yUcVqpTqpq1vFSsbUbRnZMMbxgrWCP6GbE7IO3IR4zNjrGKtOCfnUta8bssN-b0F59CPSC0OmHZLD8lPIx0Q4hzQ0m6hXUBIOwoj9Ev0kfqR4t89Bj_gmKCnvX_BkF-f57CszbRDGiC9J28d9BGvjvWS_Lr_9nP7WDz9ePi-_fpUmEo1qWg6VkopmRLCgSutkK1SLt8VMqekFc66moMSYBV2leEdlNa4qoWmyYVXl-TTIXcfpj8zxqQHHw32PYw4zVFLmfNL1WZQHEATphgDOr3Pf4CwaM70qlSvvvTqS7e1flWq17EPx_y5G9Ceho4Oc__jsQ_RQO8CjMbHE1YJLoWSGbs7YJhdvHgMOhqPo0HrA5qk7eT_v8c_FyyThA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77277289</pqid></control><display><type>article</type><title>Caffeine demethylation measured by breath analysis in experimental liver injury in the rat</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Schaad, Heinz J. ; Renner, Eberhard L. ; Wietholtz, Hubertus ; Arnaud, Maurice J. ; Preisig, Rudolf</creator><creatorcontrib>Schaad, Heinz J. ; Renner, Eberhard L. ; Wietholtz, Hubertus ; Arnaud, Maurice J. ; Preisig, Rudolf</creatorcontrib><description>To assess the effects of experimental liver injury on caffeine metabolism, 1 μCi/kg b.w. of [3-methyl
14C]-caffeine (together with 5 mg/kg b.w. of the cold compound) was injected i.p. to four different experimental groups and respective controls of unanesthetized male Sprague-Dawley rats. Exhaled
14CO
2 was completely collected during 4 h and peak exhalation rate and fraction of dose recovered were calculated.
1
3
hepatectomy affected
14CO
2 exhalation to a limited extent, decreasing solely peak exhalation rate (
p<0.05 compared to sham-operated controls).
2
3
hepatectomy, on the other hand, resulted in significant reduction (
p<0.01) in both peak exhalation rate (by 59%) and fraction of dose recovered (by 47%), that were proportionate to the loss of liver mass (59%). End-to-side portocaval shunt led to the well-documented hepatic “atrophy”, liver weight being diminished on average to 50% within 2 weeks of surgery; however, reductions in peak exhalation rate (by 75%) and fraction of dose recovered (by 64%) were even more pronounced. Finally, 48 h bile duct ligation was equivalent to “functional
2
3
hepatectomy”, peak exhalation rate (by 65%) and fraction of dose recovered (by 56%) being markedly diminished despite increased liver weight. These results indicate that
14CO
2 exhalation curves following administration of specifically labelled caffeine are quantitative indicators of acute or chronic loss of functioning liver mass. In addition, the 3-demethylation pathway appears to be particularly sensitive to the inhibitory effects of cholestasis on microsomal function.</description><identifier>ISSN: 0168-8278</identifier><identifier>EISSN: 1600-0641</identifier><identifier>DOI: 10.1016/0168-8278(95)80264-9</identifier><identifier>PMID: 7751592</identifier><identifier>CODEN: JOHEEC</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Animals ; Bile duct ligation ; Biological and medical sciences ; Breath analysis ; Breath Tests ; Caffeine - metabolism ; Caffeine - pharmacokinetics ; Caffeine demethylation ; Carbon Dioxide ; Carbon Radioisotopes ; Gastroenterology. Liver. Pancreas. Abdomen ; Hepatectomy ; Liver disease models ; Liver Diseases - metabolism ; Liver function ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Methylation ; Other diseases. Semiology ; Portocaval anastomosis ; Rat ; Rats ; Rats, Sprague-Dawley ; Respiration</subject><ispartof>Journal of hepatology, 1995, Vol.22 (1), p.82-87</ispartof><rights>1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-6b027770844faf2d47988f7088e0f87d4fdf51a84ad8eb3c1ba2dcf39a66cf313</citedby><cites>FETCH-LOGICAL-c386t-6b027770844faf2d47988f7088e0f87d4fdf51a84ad8eb3c1ba2dcf39a66cf313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-8278(95)80264-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3417487$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7751592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schaad, Heinz J.</creatorcontrib><creatorcontrib>Renner, Eberhard L.</creatorcontrib><creatorcontrib>Wietholtz, Hubertus</creatorcontrib><creatorcontrib>Arnaud, Maurice J.</creatorcontrib><creatorcontrib>Preisig, Rudolf</creatorcontrib><title>Caffeine demethylation measured by breath analysis in experimental liver injury in the rat</title><title>Journal of hepatology</title><addtitle>J Hepatol</addtitle><description>To assess the effects of experimental liver injury on caffeine metabolism, 1 μCi/kg b.w. of [3-methyl
14C]-caffeine (together with 5 mg/kg b.w. of the cold compound) was injected i.p. to four different experimental groups and respective controls of unanesthetized male Sprague-Dawley rats. Exhaled
14CO
2 was completely collected during 4 h and peak exhalation rate and fraction of dose recovered were calculated.
1
3
hepatectomy affected
14CO
2 exhalation to a limited extent, decreasing solely peak exhalation rate (
p<0.05 compared to sham-operated controls).
2
3
hepatectomy, on the other hand, resulted in significant reduction (
p<0.01) in both peak exhalation rate (by 59%) and fraction of dose recovered (by 47%), that were proportionate to the loss of liver mass (59%). End-to-side portocaval shunt led to the well-documented hepatic “atrophy”, liver weight being diminished on average to 50% within 2 weeks of surgery; however, reductions in peak exhalation rate (by 75%) and fraction of dose recovered (by 64%) were even more pronounced. Finally, 48 h bile duct ligation was equivalent to “functional
2
3
hepatectomy”, peak exhalation rate (by 65%) and fraction of dose recovered (by 56%) being markedly diminished despite increased liver weight. These results indicate that
14CO
2 exhalation curves following administration of specifically labelled caffeine are quantitative indicators of acute or chronic loss of functioning liver mass. In addition, the 3-demethylation pathway appears to be particularly sensitive to the inhibitory effects of cholestasis on microsomal function.</description><subject>Animals</subject><subject>Bile duct ligation</subject><subject>Biological and medical sciences</subject><subject>Breath analysis</subject><subject>Breath Tests</subject><subject>Caffeine - metabolism</subject><subject>Caffeine - pharmacokinetics</subject><subject>Caffeine demethylation</subject><subject>Carbon Dioxide</subject><subject>Carbon Radioisotopes</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Hepatectomy</subject><subject>Liver disease models</subject><subject>Liver Diseases - metabolism</subject><subject>Liver function</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methylation</subject><subject>Other diseases. Semiology</subject><subject>Portocaval anastomosis</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Respiration</subject><issn>0168-8278</issn><issn>1600-0641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSTdp_kEKOpSSHNxKtmzJl0BZmqQQ6KW99CLG0ohV8MdWkkP97ytnlz32IAZpnnkZPYRcc_aZM958yUcVqpTqpq1vFSsbUbRnZMMbxgrWCP6GbE7IO3IR4zNjrGKtOCfnUta8bssN-b0F59CPSC0OmHZLD8lPIx0Q4hzQ0m6hXUBIOwoj9Ev0kfqR4t89Bj_gmKCnvX_BkF-f57CszbRDGiC9J28d9BGvjvWS_Lr_9nP7WDz9ePi-_fpUmEo1qWg6VkopmRLCgSutkK1SLt8VMqekFc66moMSYBV2leEdlNa4qoWmyYVXl-TTIXcfpj8zxqQHHw32PYw4zVFLmfNL1WZQHEATphgDOr3Pf4CwaM70qlSvvvTqS7e1flWq17EPx_y5G9Ceho4Oc__jsQ_RQO8CjMbHE1YJLoWSGbs7YJhdvHgMOhqPo0HrA5qk7eT_v8c_FyyThA</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Schaad, Heinz J.</creator><creator>Renner, Eberhard L.</creator><creator>Wietholtz, Hubertus</creator><creator>Arnaud, Maurice J.</creator><creator>Preisig, Rudolf</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Caffeine demethylation measured by breath analysis in experimental liver injury in the rat</title><author>Schaad, Heinz J. ; Renner, Eberhard L. ; Wietholtz, Hubertus ; Arnaud, Maurice J. ; Preisig, Rudolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-6b027770844faf2d47988f7088e0f87d4fdf51a84ad8eb3c1ba2dcf39a66cf313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Bile duct ligation</topic><topic>Biological and medical sciences</topic><topic>Breath analysis</topic><topic>Breath Tests</topic><topic>Caffeine - metabolism</topic><topic>Caffeine - pharmacokinetics</topic><topic>Caffeine demethylation</topic><topic>Carbon Dioxide</topic><topic>Carbon Radioisotopes</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Hepatectomy</topic><topic>Liver disease models</topic><topic>Liver Diseases - metabolism</topic><topic>Liver function</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Methylation</topic><topic>Other diseases. Semiology</topic><topic>Portocaval anastomosis</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Respiration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schaad, Heinz J.</creatorcontrib><creatorcontrib>Renner, Eberhard L.</creatorcontrib><creatorcontrib>Wietholtz, Hubertus</creatorcontrib><creatorcontrib>Arnaud, Maurice J.</creatorcontrib><creatorcontrib>Preisig, Rudolf</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schaad, Heinz J.</au><au>Renner, Eberhard L.</au><au>Wietholtz, Hubertus</au><au>Arnaud, Maurice J.</au><au>Preisig, Rudolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Caffeine demethylation measured by breath analysis in experimental liver injury in the rat</atitle><jtitle>Journal of hepatology</jtitle><addtitle>J Hepatol</addtitle><date>1995</date><risdate>1995</risdate><volume>22</volume><issue>1</issue><spage>82</spage><epage>87</epage><pages>82-87</pages><issn>0168-8278</issn><eissn>1600-0641</eissn><coden>JOHEEC</coden><abstract>To assess the effects of experimental liver injury on caffeine metabolism, 1 μCi/kg b.w. of [3-methyl
14C]-caffeine (together with 5 mg/kg b.w. of the cold compound) was injected i.p. to four different experimental groups and respective controls of unanesthetized male Sprague-Dawley rats. Exhaled
14CO
2 was completely collected during 4 h and peak exhalation rate and fraction of dose recovered were calculated.
1
3
hepatectomy affected
14CO
2 exhalation to a limited extent, decreasing solely peak exhalation rate (
p<0.05 compared to sham-operated controls).
2
3
hepatectomy, on the other hand, resulted in significant reduction (
p<0.01) in both peak exhalation rate (by 59%) and fraction of dose recovered (by 47%), that were proportionate to the loss of liver mass (59%). End-to-side portocaval shunt led to the well-documented hepatic “atrophy”, liver weight being diminished on average to 50% within 2 weeks of surgery; however, reductions in peak exhalation rate (by 75%) and fraction of dose recovered (by 64%) were even more pronounced. Finally, 48 h bile duct ligation was equivalent to “functional
2
3
hepatectomy”, peak exhalation rate (by 65%) and fraction of dose recovered (by 56%) being markedly diminished despite increased liver weight. These results indicate that
14CO
2 exhalation curves following administration of specifically labelled caffeine are quantitative indicators of acute or chronic loss of functioning liver mass. In addition, the 3-demethylation pathway appears to be particularly sensitive to the inhibitory effects of cholestasis on microsomal function.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>7751592</pmid><doi>10.1016/0168-8278(95)80264-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of hepatology, 1995, Vol.22 (1), p.82-87 |
| issn | 0168-8278 1600-0641 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77277289 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bile duct ligation Biological and medical sciences Breath analysis Breath Tests Caffeine - metabolism Caffeine - pharmacokinetics Caffeine demethylation Carbon Dioxide Carbon Radioisotopes Gastroenterology. Liver. Pancreas. Abdomen Hepatectomy Liver disease models Liver Diseases - metabolism Liver function Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Methylation Other diseases. Semiology Portocaval anastomosis Rat Rats Rats, Sprague-Dawley Respiration |
| title | Caffeine demethylation measured by breath analysis in experimental liver injury in the rat |
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