Commitment and maintenance of the alpha beta and gamma delta T cell lineages
We have generated transgenic mice using a rearranged, functional TCR-gamma gene that lacks the C gamma 1 3' silencer element, together with a TCR-delta cDNA construct designed for expression in lymphoid cells. All transgenic mice that descended from nine founders expressed alpha beta T cells an...
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Veröffentlicht in: | The Journal of immunology (1950) 1995-06, Vol.154 (11), p.5821-5831 |
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Sprache: | eng |
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Zusammenfassung: | We have generated transgenic mice using a rearranged, functional TCR-gamma gene that lacks the C gamma 1 3' silencer element, together with a TCR-delta cDNA construct designed for expression in lymphoid cells. All transgenic mice that descended from nine founders expressed alpha beta T cells and elevated levels of gamma delta T cells at various ratios. This observation does not support the proposal that alpha beta T cells are generated from T precursors in which C gamma 1 genes have been repressed via the cis-acting 3' silencer element, but it supports the idea that lineage commitment occurs independently of TCR gene expression. In seven transgenic lines, despite the absence of the 3' C gamma 1 silencer on the gamma transgene, the transgenic TCR is expressed only in gamma delta and not in alpha beta T cells. In the other two lines, in addition to T cells that express either the alpha beta or gamma delta TCR, a substantial fraction of T cells expresses both alpha beta and gamma delta TCR. These two lines also carry the highest number of copies of TCR-gamma transgene. However, in general, there is no correlation between the copy number of the gamma transgene and the fraction of T cells expressing the gamma delta TCR. The implications of these data are discussed. |
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ISSN: | 0022-1767 1550-6606 |
DOI: | 10.4049/jimmunol.154.11.5821 |