Influence of 1,25-hydroxyvitamin D3 on cytosolic free calcium concentrations

Using isolated cartilage cells from the epiphyseal growth plate of rachitic chicks and utilizing the fluorescence indicator quin2 for measurements of cytosolic free calcium, it has been possible to demonstrate that 1,25-dihydroxyvitamin D3 regulates the concentrations of cytosolic free calcium. The...

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Veröffentlicht in:	Biochemical and biophysical research communications 1986-12, Vol.141 (3), p.1236-1241
Hauptverfasser:	Bar, S, Lidor, C, Harell, A, Edelstein, S
Format:	Artikel
Sprache:	eng
Schlagworte:	1,25-DIHYDROXYCHOLECALCIFEROL Alkaline Phosphatase - antagonists & inhibitors Aminoquinolines Animals Applied sciences Calcimycin - pharmacology CALCIO Calcitriol - pharmacology CALCIUM Calcium - metabolism Chickens CHICKS Cytosol - metabolism Exact sciences and technology Fluorescent Dyes Growth Plate - drug effects Growth Plate - metabolism Other techniques and industries POLLITO POUSSIN RACHITISME RAQUITISMO RICKETS Rickets - metabolism Spectrometry, Fluorescence VITAMIN D VITAMINA D VITAMINE D
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Zusammenfassung:	Using isolated cartilage cells from the epiphyseal growth plate of rachitic chicks and utilizing the fluorescence indicator quin2 for measurements of cytosolic free calcium, it has been possible to demonstrate that 1,25-dihydroxyvitamin D3 regulates the concentrations of cytosolic free calcium. The reduction in cytosolic free calcium is associated with inhibition of the activity of alkaline phosphatase. As several hours are required before an effect on cytosolic free calcium can be observed in the cells, it is suggested that the action is dependent on genomic interactions.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(86)80177-2