Sequential volume changes on a small sample by density

A procedure is described for generating a sequence of volume changes by density on the same sample of a solution. The entire volume-change profile over the reactant concentration range of interest can be accomplished with 0.1–0.2 μmol of a protein in 100 μl of sample. The calcium-binding proteins, c...

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Veröffentlicht in:	Analytical biochemistry 1986-11, Vol.158 (2), p.463-468
1. Verfasser:	Kupke, Donald W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Applied sciences Biological and medical sciences Calcium - metabolism calcium-binding proteins Calcium-Binding Proteins - metabolism Calmodulin - metabolism Cattle density Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation methods instrumentation Microchemistry Other techniques and industries protein structure Proteins Rabbits Solutions Thermodynamics Troponin - metabolism Troponin C volume change
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Beschreibung
Zusammenfassung:	A procedure is described for generating a sequence of volume changes by density on the same sample of a solution. The entire volume-change profile over the reactant concentration range of interest can be accomplished with 0.1–0.2 μmol of a protein in 100 μl of sample. The calcium-binding proteins, calmodulin and skeletal troponin-C, were employed to determine the volume increases attending the sequential addition of calcium ions. Both volume-change profiles exhibited nonlinear increases over the first four equivalents of calcium ion added.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(86)90576-2