Recovery of nanogram quantities of DNA from plasma and quantitative measurement using labeling by nick translation

A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1986-11, Vol.158 (2), p.250-256
Hauptverfasser:	FOURNIE, G. J, GAYRAL-TAMINH, M, BOUCHE, J.-P, CONTE, J. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Applied sciences bacterial lipopolysaccharide Biological and medical sciences DNA DNA - biosynthesis DNA - blood Dna, deoxyribonucleoproteins ethanol precipitation of DNA Exact sciences and technology Fundamental and applied biological sciences. Psychology Gelatin Kinetics Lipopolysaccharides - pharmacology Mice Microchemistry - methods micromethods nick translation Nucleic acids Other techniques and industries plasma plasma DNA Polyethylene Glycols systemic lupus erythematosus Temperature translation
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method which allows for the quantitative measurement of DNA in plasma is described. After treatment of plasma with phenol, DNA is precipitated by ethanol using gelatin as a coprecipitating agent. DNA is then measured by nick translation labeling. This assay takes a few hours. It is suitable for the measurement of DNA within a range of 0.02 to 20 ng in 10 μl of plasma. For example, it is applied to the measurement of DNA in plasma from mice injected with bacterial lipopolysaccharide.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(86)90545-2