Human Foreskin Mast Cell Viability and Functional Activity Is Maintained ex Vivo by Coculture with Fibroblasts
The aim of the present study was to develop long-term culture conditions for foreskin-derived mast cells (HSMC) as we have previously done for rodent peritoneal mast cells (MC) and human lung-derived MC. HSMC were obtained by proteolytic treatment of foreskins from 8-day-old babies (4.8 ± 2.0% purit...
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Veröffentlicht in: | Cellular immunology 1995-05, Vol.162 (2), p.211-216 |
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Sprache: | eng |
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Zusammenfassung: | The aim of the present study was to develop long-term culture conditions for foreskin-derived mast cells (HSMC) as we have previously done for rodent peritoneal mast cells (MC) and human lung-derived MC. HSMC were obtained by proteolytic treatment of foreskins from 8-day-old babies (4.8 ± 2.0% purity) and seeded onto a confluent monolayer of human foreskin-derived fibroblasts (HF) in enriched culture medium. HSMC biochemical and functional properties were studied up to 8 days in these cocultures. Twenty-four hours after seeding the cell suspensions from the proteolytic-digested foreskins, all the cells which did not adhere to the HF were washed out. At this time point ∼ 30% of the seeded HSMC were found to adhere to the HF monolayer. The only contaminating cells were endothelial cells ( |
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ISSN: | 0008-8749 1090-2163 |
DOI: | 10.1006/cimm.1995.1071 |