Screening and sequence determination of a cDNA encoding the human brain 4-aminobutyrate aminotransferase

Screening and sequence determination of a cDNA encoding the human brain 4-aminobutyrate aminotransferase

A human brain cDNA library constructed in the λZAP®II vector was screened using a fragment of pig brain cDNA encoding 4-aminobutyrate aminotransferase (pGaba-t). A cDNA that encodes the human brain Gaba-t (hGaba-t) has been isolated from the library and sequenced. Using the GenBank and EMBL database...

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Veröffentlicht in:Gene 1995-04, Vol.155 (2), p.185-187
Hauptverfasser: Osei, Yaa D., Churchich, Jorge E.
Format: Artikel
Sprache:eng
Schlagworte:
4-Aminobutyrate Transaminase - genetics
Amino Acid Sequence
Animals
Base Sequence
Brain - enzymology
DNA, Complementary - genetics
GABA
Humans
Inhibitory neurotransmitter
Molecular Sequence Data
phage λZAP®II vector
rescued phagemid
Sequence Alignment
Sequence Analysis
Swine
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Beschreibung
Zusammenfassung:A human brain cDNA library constructed in the λZAP®II vector was screened using a fragment of pig brain cDNA encoding 4-aminobutyrate aminotransferase (pGaba-t). A cDNA that encodes the human brain Gaba-t (hGaba-t) has been isolated from the library and sequenced. Using the GenBank and EMBL databases, comparison of the predicted amino-acid sequence of hGaba-t with the pig enzyme revealed 95.4% homology.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)00858-P