Characterisation of potential regulatory elements within the rat preprotachykinin-A promoter
The rat preprotachykinin-A (rPPT-A) gene encodes the precursor of several tachykinin neuropeptides including substance P. Previous studies have demonstrated the presence of multiple DNA sequences important for directing expression of the rPPT-A gene in dorsal root ganglion neurons within a region of...
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Veröffentlicht in: | Neuroscience letters 1995-01, Vol.184 (2), p.125-128 |
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Sprache: | eng |
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Zusammenfassung: | The rat preprotachykinin-A (rPPT-A) gene encodes the precursor of several tachykinin neuropeptides including substance P. Previous studies have demonstrated the presence of multiple DNA sequences important for directing expression of the rPPT-A gene in dorsal root ganglion neurons within a region of the promoter spanning nucleotides −865 and −47. In order to identify potential
cis acting elements, we have carried out DNase 1 footprinting analysis using a series of constructs containing fragments from this region of the promoter. This study has defined three potential AP-1 complex interactions, two E box binding protein interactions and two dG rich elements, which are potentially bound by complexes related to AP-2 or Sp1 in this region of the promoter. |
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ISSN: | 0304-3940 1872-7972 |
DOI: | 10.1016/0304-3940(94)11186-M |