Determination of Lysine with Ninhydrin-Ferric Reagent

Ninhydrin-ferric reagent reacts highly and specifically with lysine at a pH value of 1.0. Ferric ion inhibits the reaction of ninhydrin with proline, ornithine, glycine, arginine, and histidine. This method can be used to determine a lysine sample of high concentration without dilution. It has a lin...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1995, Vol.224 (1), p.187-189
Hauptverfasser:	Hsieh, C.L., Hsiung, K.P., Su, J.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ferric Compounds Hydrogen-Ion Concentration Indicators and Reagents Lysine - analysis Ninhydrin Sensitivity and Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Ninhydrin-ferric reagent reacts highly and specifically with lysine at a pH value of 1.0. Ferric ion inhibits the reaction of ninhydrin with proline, ornithine, glycine, arginine, and histidine. This method can be used to determine a lysine sample of high concentration without dilution. It has a linear response range from 0.0625 to 0.5 mg lysine·HCl.
ISSN:	0003-2697 1096-0309
DOI:	10.1006/abio.1995.1027