Expression of the rabbit sperm protein Sp17 in cos cells and interaction of recombinant Sp17 with the rabbit zona pellucida

Expression of the rabbit sperm protein Sp17 in cos cells and interaction of recombinant Sp17 with the rabbit zona pellucida

This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the cross‐linking, reagent DSS that rSp17 can...

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Veröffentlicht in:Molecular reproduction and development 1995-01, Vol.40 (1), p.48-55
Hauptverfasser: O'rand, Michael G., Richardson, Richard T., Yamasaki, Noriyuki
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Autoantigens - genetics
Autoantigens - metabolism
Base Sequence
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - immunology
Carrier Proteins - metabolism
Cell Line
COS cell expression
DNA Primers - genetics
Egg Proteins - metabolism
Female
Fertilization
Fundamental and applied biological sciences. Psychology
Gene Expression
In Vitro Techniques
Male
Mammalian male genital system
Membrane Glycoproteins - metabolism
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Molecular Sequence Data
Morphology. Physiology
Protein Binding
Rabbits
Receptors, Cell Surface
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Recombinant Proteins - metabolism
Sperm protein
Transfection
Vertebrates: reproduction
Zona binding protein
Zona pellucida
Zona Pellucida - metabolism
Zona Pellucida Glycoproteins
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Zusammenfassung:This study extends our analysis of rabbit recombinant Sp17 (rSp17) by examining whether rSp17 synthesized in transfected COS cells will show a particular localization within the cell and whether the COS cell will bind with zona pellucida. We show, using the cross‐linking, reagent DSS that rSp17 can bind to rabbit zona glycoprotein R45 or R55. © 1995 Wiley‐Liss, Inc.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.1080400107