Cytochrome C Oxidase in Alzheimer's Disease Brain: Purification and Characterization

Article abstract-Cytochrome c oxidase (COX) is deficient in both peripheral tissue and brain of Alzheimer's disease (AD) patients and may be of pathogenic significance in AD. We purified COX from AD brains (n = 3) and control brains (n = 3) and characterized the enzyme kinetically and spectrall...

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Veröffentlicht in:	Neurology 1995-03, Vol.45 (3), p.482-486
Hauptverfasser:	Parker, W Davis, Parks, Janice K
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Aged, 80 and over Alzheimer Disease - enzymology Binding Sites Biological and medical sciences Brain - enzymology Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Electron Transport Complex IV - isolation & purification Electron Transport Complex IV - metabolism Humans Kinetics Medical sciences Middle Aged Neurology Polarography Spectrophotometry
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Zusammenfassung:	Article abstract-Cytochrome c oxidase (COX) is deficient in both peripheral tissue and brain of Alzheimer's disease (AD) patients and may be of pathogenic significance in AD. We purified COX from AD brains (n = 3) and control brains (n = 3) and characterized the enzyme kinetically and spectrally. Purified AD brain COX displayed anomalous kinetic behavior compared with control brain COX in that the low Km binding site was kinetically unidentifiable. For purposes of comparison, we purified COX from a standard beef heart preparation and found normal kinetic behavior. AD brain COX may be structurally abnormal and may make an important contribution to the bioenergetic defect seen in AD. NEUROLOGY 1995;45482-486
ISSN:	0028-3878 1526-632X
DOI:	10.1212/WNL.45.3.482