Regulation of mouse myelin basic protein gene transcription by a sequence-specific single-stranded DNA-binding protein in vitro
Cell- and stage-specific transcription of the myelin basic protein (MBP)-encoding gene ( Mbp) in brain is regulated by arrays of cis-acting regulatory elements positioned at the 5'-flanking region of the gene. The proximal element between nucleotides - 14 to - 50, termed MBI, has previously bee...
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Veröffentlicht in: | Gene 1995-01, Vol.154 (2), p.215-218 |
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Zusammenfassung: | Cell- and stage-specific transcription of the myelin basic protein (MBP)-encoding gene (
Mbp) in brain is regulated by arrays of
cis-acting regulatory elements positioned at the 5'-flanking region of the gene. The proximal element between nucleotides - 14 to - 50, termed
MBI, has previously been shown to have an important role in the cell-type-specific transcription of
Mbp in cells derived from the central nervous system (CNS). Here, we utilized band-shift and in vitro transcription assays to examine the ability of MEF-2, an expression factor encoded by a cDNA isolated from mouse brain, in binding to the
MB1 element and regulating transcription of the
Mbp promoter. Results from the bandshift assays indicated that the bacterially produced MEF-2 recognizes specific nt in the
MB1 motif, and its binding to these nt reduces the overall transcriptional activity of
Mbp in a cell-free extract. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/0378-1119(94)00816-B |