Regulation of mouse myelin basic protein gene transcription by a sequence-specific single-stranded DNA-binding protein in vitro

Cell- and stage-specific transcription of the myelin basic protein (MBP)-encoding gene ( Mbp) in brain is regulated by arrays of cis-acting regulatory elements positioned at the 5'-flanking region of the gene. The proximal element between nucleotides - 14 to - 50, termed MBI, has previously bee...

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Veröffentlicht in:Gene 1995-01, Vol.154 (2), p.215-218
Hauptverfasser: Steplewski, Andrzej, Haas, Susan, Amini, Shohreh, Khalili, Kamel
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Sprache:eng
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Zusammenfassung:Cell- and stage-specific transcription of the myelin basic protein (MBP)-encoding gene ( Mbp) in brain is regulated by arrays of cis-acting regulatory elements positioned at the 5'-flanking region of the gene. The proximal element between nucleotides - 14 to - 50, termed MBI, has previously been shown to have an important role in the cell-type-specific transcription of Mbp in cells derived from the central nervous system (CNS). Here, we utilized band-shift and in vitro transcription assays to examine the ability of MEF-2, an expression factor encoded by a cDNA isolated from mouse brain, in binding to the MB1 element and regulating transcription of the Mbp promoter. Results from the bandshift assays indicated that the bacterially produced MEF-2 recognizes specific nt in the MB1 motif, and its binding to these nt reduces the overall transcriptional activity of Mbp in a cell-free extract.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)00816-B