Isolation and Structural-Analysis of the 5′ Flanking Region of the Gene Encoding the Human Glucagon Receptor
The gene encoding the human glucagon receptor, including several kb of upstream sequence, was isolated from a bacteriophage λ FIX II library constructed from human placental DNA. We report here the novel sequence of the 5′ flanking region of the gene, the identification of a previously unreported in...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-03, Vol.208 (1), p.339-344 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Buggy, J. Hull, J. Yoowarren, H. |
| description | The gene encoding the human glucagon receptor, including several kb of upstream sequence, was isolated from a bacteriophage λ FIX II library constructed from human placental DNA. We report here the novel sequence of the 5′ flanking region of the gene, the identification of a previously unreported intron of 5 kb, and the identification of the transcription start point of the glucagon receptor-specific transcript, which estimates the length of the first exon to be 300 bp. |
| doi_str_mv | 10.1006/bbrc.1995.1343 |
| format | Article |
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| ispartof | Biochemical and biophysical research communications, 1995-03, Vol.208 (1), p.339-344 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Base Sequence DNA - genetics DNA - metabolism DNA Primers Female Gene Library Humans Molecular Sequence Data Placenta - metabolism Polymerase Chain Reaction Pregnancy Promoter Regions, Genetic Receptors, Glucagon - biosynthesis Receptors, Glucagon - genetics Restriction Mapping RNA, Messenger - analysis RNA, Messenger - biosynthesis Transcription, Genetic |
| title | Isolation and Structural-Analysis of the 5′ Flanking Region of the Gene Encoding the Human Glucagon Receptor |
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