Isolation and Structural-Analysis of the 5′ Flanking Region of the Gene Encoding the Human Glucagon Receptor

The gene encoding the human glucagon receptor, including several kb of upstream sequence, was isolated from a bacteriophage λ FIX II library constructed from human placental DNA. We report here the novel sequence of the 5′ flanking region of the gene, the identification of a previously unreported intron of 5 kb, and the identification of the transcription start point of the glucagon receptor-specific transcript, which estimates the length of the first exon to be 300 bp.