Single-step immunization using a controlled release, biodegradable polymer with sustained adjuvant activity

The use of a biodegradable polymer for antigen delivery based on poly(CTTH-iminocarbonate) (IUPAC nomenclature: poly[oxyimidocarbonyloxy- p-phenylene[2-(hexyloxycarbonyl)ethylene]imino[2-[1-(benzyloxy)formamido]-1-oxotrimethylene]- p- phenylene]) was investigated. This polymer was selected since its primary degradation product, N- benzyloxycarbonyl- L-tyrosine hexyl ester (CTTH) was found to be as potent an adjuvant as complete Freund's adjuvant and muramyl dipeptide, when measuring the serum antibody response to bovine serum albumin (BSA) in male CD-1 mice over 56 weeks. BSA released from subcutaneously implanted polymeric antigen delivery devices made of poly(CTTH-iminocarbonate) resulted in significantly higher ( P < 0.006) anti-BSA antibody titers than devices made of poly(bisphenol A-iminocarbonate).